Characterization of the pilin gene of Moraxella bovis Dalton 2d and expression of pili from M. bovis in Pseudomonas aeruginosa
| Autor: | T. C. Elleman, Peter A. Hoyne, A. W. D. Lepper |
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| Rok vydání: | 1990 |
| Předmět: |
Blotting
Western Molecular Sequence Data Immunology Fimbria Moraxella bovis Gene Expression Cell Fractionation Microbiology Pilus Fimbriae Proteins Escherichia coli Moraxella Amino Acid Sequence Cloning Molecular Peptide sequence Base Sequence biology Nucleic acid sequence biology.organism_classification Molecular biology Infectious Diseases Genes Bacterial Fimbriae Bacterial Pilin Pseudomonas aeruginosa biology.protein bacteria Parasitology DNA Probes Research Article Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and Immunity. 58:1678-1684 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | The pilin gene of Moraxella bovis Dalton 2d was isolated by cloning in Pseudomonas aeruginosa. The nucleotide sequence of this gene encodes a prepilin of 156 amino acid residues. When high levels of pilin were expressed from the gene in P. aeruginosa, by using the pL promoter of bacteriophage lambda inserted upstream of the coding sequence, pili which were indistinguishable from pili of M. bovis were produced. |
| Databáze: | OpenAIRE |
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