Genome-wide annotation, expression profiling, and protein interaction studies of the core cell-cycle genes in Phalaenopsis aphrodite
| Autor: | Miao-Ju Wei, Zin-Huang Liu, Su-Chiung Fang, Huang-Hsien Li, Hsiang-Yin Lin, Swee-Suak Ko, Yi-Chen Lien, Jhun-Chen Chen |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Phalaenopsis aphrodite
Meristematic tissues Molecular Sequence Data Cell Cycle Proteins Plant Science Cell cycle Genome Article Transcriptome Gene Expression Regulation Plant Cyclin-dependent kinase Botany Orchid Genetics Amino Acid Sequence Orchidaceae Gene Phylogeny Plant Proteins biology Protocorm General Medicine biology.organism_classification Cell Cycle Gene Gene expression profiling Evolutionary biology Multigene Family biology.protein Phalaenopsis Agronomy and Crop Science Genome Plant |
| Zdroj: | Plant Molecular Biology |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/s11103-013-0128-y |
| Popis: | Orchidaceae is one of the most abundant and diverse families in the plant kingdom and its unique developmental patterns have drawn the attention of many evolutionary biologists. Particular areas of interest have included the co-evolution of pollinators and distinct floral structures, and symbiotic relationships with mycorrhizal flora. However, comprehensive studies to decipher the molecular basis of growth and development in orchids remain scarce. Cell proliferation governed by cell-cycle regulation is fundamental to growth and development of the plant body. We took advantage of recently released transcriptome information to systematically isolate and annotate the core cell-cycle regulators in the moth orchid Phalaenopsis aphrodite. Our data verified that Phalaenopsis cyclin-dependent kinase A (CDKA) is an evolutionarily conserved CDK. Expression profiling studies suggested that core cell-cycle genes functioning during the G1/S, S, and G2/M stages were preferentially enriched in the meristematic tissues that have high proliferation activity. In addition, subcellular localization and pairwise interaction analyses of various combinations of CDKs and cyclins, and of E2 promoter-binding factors and dimerization partners confirmed interactions of the functional units. Furthermore, our data showed that expression of the core cell-cycle genes was coordinately regulated during pollination-induced reproductive development. The data obtained establish a fundamental framework for study of the cell-cycle machinery in Phalaenopsis orchids. Electronic supplementary material The online version of this article (doi:10.1007/s11103-013-0128-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink