Mechanical Ring Interfaces between Adherens Junction and Contractile Actomyosin to Coordinate Entotic Cell-in-Cell Formation
| Autor: | Xiangkai Ning, Xiaoning Wang, Manna Wang, Hongquan Qin, Qiang Sun, Songzhi Gu, Li Ma, Zubiao Niu, You Zheng, Banzhan Ruan, Hongyan Huang, Lihua Gao, Zhaolie Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Entosis media_common.quotation_subject Cell mechanical ring adherens junction General Biochemistry Genetics and Molecular Biology Adherens junction 03 medical and health sciences 0302 clinical medicine medicine Humans cell-in-cell structures Cytoskeleton Internalization lcsh:QH301-705.5 media_common biology entosis Chemistry vinculin Depolarization Actomyosin Adherens Junctions Vinculin Cell-in-Cell Formation 030104 developmental biology Förster resonance energy transfer medicine.anatomical_structure cell death lcsh:Biology (General) Biophysics biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 32, Iss 8, Pp 108071-(2020) |
| ISSN: | 2211-1247 |
| Popis: | Summary: Entosis is a cell-in-cell (CIC)-mediated death program. Contractile actomyosin (CA) and the adherens junction (AJ) are two core elements essential for entotic CIC formation, but the molecular structures interfacing them remain poorly understood. Here, we report the characterization of a ring-like structure interfacing between the peripheries of invading and engulfing cells. The ring-like structure is a multi-molecular complex consisting of adhesive and cytoskeletal proteins, in which the mechanical sensor vinculin is highly enriched. The vinculin-enriched structure senses mechanical force imposed on cells, as indicated by fluorescence resonance energy transfer (FRET) analysis, and is thus termed the mechanical ring (MR). The MR actively interacts with CA and the AJ to help establish and maintain polarized actomyosin that drives cell internalization. Vinculin depletion leads to compromised MR formation, CA depolarization, and subsequent CIC failure. In summary, we suggest that the vinculin-enriched MR, in addition to CA and AJ, is another core element essential for entosis. |
| Databáze: | OpenAIRE |
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