Development and validation of HPLC method with fluorometric detection for quantification of bisnaphthalimidopropyldiaminooctane in animal tissues following administration in polymeric nanoparticles
| Autor: | Sofia A. Costa Lima, Vera L.R.G. Abreu, Anabela Cordeiro-da-Silva, Sónia Nogueira, Paul Kong Thoo Lin, Marcela A. Segundo, Marcelo V. Osório |
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| Přispěvatelé: | Instituto de Investigação e Inovação em Saúde |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Naphthalimides/metabolism Biodistribution Bioanalysis Fluorometry/standards Monolithic HPLC column Nanoparticles/metabolism Calibration curve Clinical Biochemistry Pharmaceutical Science Nanoparticles/analysis 02 engineering and technology Chromatography High Pressure Liquid/standards 01 natural sciences Fluorescence spectroscopy Analytical Chemistry Tissue Distribution/physiology chemistry.chemical_compound Acetic acid Mice Naphthalimides/administration & dosage Naphthalimides/analysis Drug Discovery Animals Fluorometry Tissue Distribution Tissue Distribution/drug effects Acetonitrile Fluorometry/methods Chromatography High Pressure Liquid Spectroscopy Mice Inbred BALB C Chromatography 010401 analytical chemistry Reproducibility of Results 021001 nanoscience & nanotechnology Nanoparticles/administration & dosage 0104 chemical sciences Naphthalimides PLGA chemistry Nanoparticles 0210 nano-technology Chromatography High Pressure Liquid/methods |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | A simple, sensitive and specific high-performance liquid chromatography method for the quantification of bisnaphthalimidopropyldiaminooctane (BNIPDaoct), a potent anti-Leishmania compound, incorporated into poly(D,L-lactide-co-glycolic acid) (PLGA) nanoparticles was developed and validated toward bioanalysis application. Biological tissue extracts were injected into a reversed-phase monolithic column coupled to a fluorimetric detector (¿ exc =234nm, ¿ em =394nm), using isocratic elution with aqueous buffer (acetic acid/acetate 0.10M, pH 4.5, 0.010M octanesulfonic acid) and acetonitrile, 60:40 (v/v) at a flow rate of 1.5mLmin -1 . The run time was 6min, with a BNIPDaoct retention time of 3.3min.Calibration curves were linear for BNIPDaoct concentrations ranging from 0.002 to 0.100µM. Matrix effects were observed and calibration curves were performed using the different organ (spleen, liver, kidney, heart and lung) extracts. The method was found to be specific, accurate (97.3-106.8% of nominal values) and precise for intra-day (RSD < 1.9%) and inter-day assays (RSD < 7.2%) in all matrices. Stability studies showed that BNIPDaoct was stable in all matrices after standing for 24h at room temperature (20°C) or in the autosampler, and after three freeze-thaw cycles. Mean recoveries of BNIPDaoct spiked in mice organs were < 88.4%. The LOD and LOQ for biological matrices were =0.8 and =1.8nM, respectively, corresponding to values =4 and =9nmolg -1 in mice organs. The method developed was successfully applied to biodistribution assessment following intravenous administration of BNIPDaoct in solution or incorporated in PLGA nanoparticles. Authors acknowledge financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT) through project UID/Multi/04378/2013, from the European Community's Seventh Framework Programme under grant agreement No. 602773 (Project KINDRED), from U.Porto/Santander Totta-Project 155/2010 and from FEDER funds under the framework of QREN through Project NORTE-07-0162-FEDER-000124. |
| Databáze: | OpenAIRE |
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