The Clearance and Degradation of Oligodeoxynucleotides Following Intravenous Injection into Rabbits
| Autor: | Byung K. Kim, Paul C. Zamecnik, John Goodchild |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Time Factors
Chromatography Base Sequence Human blood Metabolic Clearance Rate Chemistry Oligonucleotide Molecular Sequence Data Rabbit (nuclear engineering) Urine Molecular biology humanities Kinetics Oligodeoxyribonucleotides Injections Intravenous Genetics Animals Degradation (geology) Electrophoresis Polyacrylamide Gel Rabbits Clearance |
| Zdroj: | Antisense Research and Development. 1:153-160 |
| ISSN: | 1050-5261 |
| DOI: | 10.1089/ard.1991.1.153 |
| Popis: | A 32P-labeled oligodeoxynucleotide of 20 bases was found to be quite stable in freshly drawn rabbit or human blood and no degradation was apparent within 2 hr. In contrast, following injection into rabbits, the same oligodeoxynucleotide was cleared from the blood rapidly and at least 17% was completely degraded within 5 min. After 90 min, 16% of the oligonucleotide was found largely intact in the urine. At this time, radioisotope was also found in all the solid tissues tested but probably not as intact oligonucleotide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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