pH as a Driver for Ammonia-Oxidizing Archaea in Forest Soils

Autor: James I. Prosser, Marion Schrumpf, Ingo Schöning, Graeme W. Nicol, Marion Engel, Gerhard Welzl, Tesfaye Wubet, Michael Schloter, François Buscot, Brigitte Schloter-Hai, Cécile Gubry-Rangin, Thomas Rattei, Barbara Stempfhuber, Susanne Kublik, Doreen Fischer, Ganna Neskovic-Prit
Přispěvatelé: Helmholtz-Zentrum München (HZM), Max Planck Institute for Biogeochemistry (MPI-BGC), Max-Planck-Gesellschaft, Institute of Biological and Environmental Sciences [Aberdeen], University of Aberdeen, University of Vienna [Vienna], Res Unit Environm Genom, Ampère, Département Bioingénierie (BioIng), Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Synthetic and Systems Biology Unit [Szeged], Biological Research Centre [Szeged] (BRC), German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, The work has been (partly) funded by the DFG Priority Program 1374 'Infrastructure-Biodiversity-Exploratories'. Fieldwork permits were issued by the responsible state environmental offices of Baden-Württemberg, Thüringen, and Brandenburg (according to § 72 BbgNatSchG)
Rok vydání: 2014
Předmět:
Zdroj: Microbial Ecology
Microbial Ecology, Springer Verlag, 2015, 69 (4), pp.879-883. ⟨10.1007/s00248-014-0548-5⟩
ISSN: 1432-184X
0095-3628
Popis: In this study, we investigated the impact of soil pH on the diversity and abundance of archaeal ammonia oxidizers in 27 different forest soils across Germany. DNA was extracted from topsoil samples, the amoA gene, encoding ammonia monooxygenase, was amplified; and the amplicons were sequenced using a 454-based pyrosequencing approach. As expected, the ratio of archaeal (AOA) to bacterial (AOB) ammonia oxidizers' amoA genes increased sharply with decreasing soil pH. The diversity of AOA differed significantly between sites with ultra-acidic soil pH (3.5) and sites with higher pH values. The major OTUs from soil samples with low pH could be detected at each site with a soil pH3.5 but not at sites with pH4.5, regardless of geographic position and vegetation. These OTUs could be related to the Nitrosotalea group 1.1 and the Nitrososphaera subcluster 7.2, respectively, and showed significant similarities to OTUs described from other acidic environments. Conversely, none of the major OTUs typical of sites with a soil pH4.6 could be found in the ultra- and extreme acidic soils. Based on a comparison with the amoA gene sequence data from a previous study performed on agricultural soils, we could clearly show that the development of AOA communities in soils with ultra-acidic pH (3.5) is mainly triggered by soil pH and is not influenced significantly by the type of land use, the soil type, or the geographic position of the site, which was observed for sites with acido-neutral soil pH.
Databáze: OpenAIRE
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