A modular approach to enzymatic ligation of peptides and proteins with oligonucleotides
| Autor: | Anh Tuân Phan, Derrick Jing Yang Tan, Vee Vee Cheong, Kah Wai Lim |
|---|---|
| Přispěvatelé: | School of Physical and Mathematical Sciences, NTU Institute of Structural Biology |
| Rok vydání: | 2021 |
| Předmět: |
Oligonucleotides
Peptide Computational biology 010402 general chemistry 01 natural sciences Catalysis 03 medical and health sciences Organophosphorus Compounds Bacterial Proteins Chemistry [Science] Materials Chemistry Amino Acid Sequence Solid-Phase Synthesis Techniques 030304 developmental biology chemistry.chemical_classification 0303 health sciences Base Sequence business.industry Oligonucleotide Metals and Alloys Biological sciences [Science] Proteins General Chemistry Modular design Aminoacyltransferases 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Cysteine Endopeptidases Enzyme chemistry Ceramics and Composites business Ligation Peptides |
| Zdroj: | Chemical communications (Cambridge, England). 57(45) |
| ISSN: | 1364-548X |
| Popis: | Joining peptides and oligonucleotides offers potential benefits, but current methods remain laborious. Here we present a novel approach towards enzymatic ligation of the two modalities through the development of tag phosphoramidites that readily couple onto oligonucleotides. This simple and highly efficient approach paves the way towards streamlined development and production of peptide/protein-oligonucleotide conjugates. Nanyang Technological University National Research Foundation (NRF) Submitted/Accepted version This research was supported by Singapore National Research Foundation (NRF-NRFI2017-09) and Nanyang Technological University. |
| Databáze: | OpenAIRE |
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