The effects of interleukin-1 receptor antagonist protein (IRAP) infusion following spinal cord transection in rats
Autor: | John B. Gelderd, Nicholas Hall, Maureen P. O'Grady, Cecilia Ferrer, Julie A. Anderson, Janelle Oliver |
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Rok vydání: | 1996 |
Předmět: |
Male
medicine.medical_specialty medicine.drug_class Sialoglycoproteins medicine.medical_treatment Biology Lymphocyte Activation Rats Sprague-Dawley chemistry.chemical_compound Spinal cord transection Corticosterone Internal medicine medicine Splenocyte Animals Infusions Intravenous Molecular Biology Spinal Cord Injuries General Neuroscience Body Weight beta-Endorphin Laminectomy Temperature Receptors Interleukin-1 Organ Size Spinal cord Receptor antagonist Rats Interleukin 1 Receptor Antagonist Protein medicine.anatomical_structure Endocrinology chemistry Anesthesia Neurology (clinical) Astrocyte |
Zdroj: | Molecular and Chemical Neuropathology. 27:167-183 |
ISSN: | 1044-7393 |
DOI: | 10.1007/bf02815092 |
Popis: | A laminectomy was performed at the T5-T6 vertebral level in adult, male, Sprague-Dawley rats and the spinal cord transected with a scalpel. A group of sham animals was subjected to the same surgery without the transection step. A group of unhandled control rats was also included. A subgroup of transected animals received a subcutaneous osmotic minipump that dispensed IL-1 receptor antagonist protein (IRAP) at the transection site for 7 consecutive days. Another transected subgroup received a minipump that infused the vehicle only. IRAP-treated rats displayed a significant reduction in body temperature (p0.05) compared with vehicle-treated rats. The IRAP-treated rats were also less active when assessed for locomotor behavior using an HVS computerized tracking system (p0.01). IRAP treatment had no effect on serum corticosterone, beta-endorphin levels, Con A, PHA, or LPS-induced splenocyte mitogenesis when compared with vehicle-treated animals. However, half of the IRAP-treated animals exhibited a substantive reduction in the number of reactive astrocytes near the transection site, suggesting a possible effect of IRAP on astrocyte activation. |
Databáze: | OpenAIRE |
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