Isolation and characterization of antisuppressor mutations in Escherichia coli
| Autor: | M A Sullivan, R M Bock |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Mutant
Nonsense mutation Biology Lac repressor RNA Transfer Amino Acyl medicine.disease_cause Microbiology Pseudouridine chemistry.chemical_compound Suppression Genetic RNA Transfer Transduction Genetic medicine Tn10 Escherichia coli Codon Molecular Biology Genetics Mutation Base Sequence Ribosomal Protein S9 Escherichia coli Proteins beta-Galactosidase Molecular biology chemistry Lac Operon Genes Bacterial Transfer RNA bacteria Research Article |
| Zdroj: | Journal of bacteriology. 161(1) |
| ISSN: | 0021-9193 |
| Popis: | Nonsense mutations in lacI have been shown to be useful as indicators of the efficiency of nonsense suppression. From strains containing supE and a lacI nonsense mutation, selection for LacI- mutants has resulted in the isolation of four antisuppressor mutations. Tn10 insertions linked to these mutations were isolated and used to group the four mutations into three loci. The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors. The asuB3 mutation was mapped by P1 cotransduction to rpsL but does not confer resistance to streptomycin. The asuC4 mutation reduced suppression by supE by 95% and was shown biochemically to result in the loss of two pseudouridine modifications from the 3' side of the anticodon stem and loop of tRNA2Gln. This mutation is linked to purF, suggesting that it is a new allele of hisT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|