Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
Autor: | Rodrigo de Almeida Vaucher, Diogenes Dezen, Amauri Braga Simonetti, Fernando Rosado Spilki, Paulo Michel Roehe |
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Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: | |
Zdroj: | Brazilian Journal of Microbiology, Vol 42, Iss 4, Pp 1440-1444 (2011) Brazilian Journal of Microbiology, Volume: 42, Issue: 4, Pages: 1440-1444, Published: DEC 2011 Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Brazilian Journal of Microbiology v.42 n.4 2011 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
ISSN: | 1678-4405 1517-8382 |
Popis: | Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3. |
Databáze: | OpenAIRE |
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