Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Autor: Rodrigo de Almeida Vaucher, Diogenes Dezen, Amauri Braga Simonetti, Fernando Rosado Spilki, Paulo Michel Roehe
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Brazilian Journal of Microbiology, Vol 42, Iss 4, Pp 1440-1444 (2011)
Brazilian Journal of Microbiology, Volume: 42, Issue: 4, Pages: 1440-1444, Published: DEC 2011
Repositório Institucional da UFRGS
Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
Brazilian Journal of Microbiology v.42 n.4 2011
Brazilian Journal of Microbiology
Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
ISSN: 1678-4405
1517-8382
Popis: Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
Databáze: OpenAIRE