Enhanced tolerance of Cupriavidus necator NCIMB 11599 to lignocellulosic derived inhibitors by inserting NAD salvage pathway genes

Autor: Sun Mi Lee, Do-Hyun Cho, Hee Ju Jung, Byungchan Kim, Su Hyun Kim, Shashi Kant Bhatia, Ranjit Gurav, Jong-Min Jeon, Jeong-Jun Yoon, Jeong-Hoon Park, Jung-Ho Park, Yun-Gon Kim, Yung-Hun Yang
Rok vydání: 2022
Předmět:
Zdroj: Bioprocess and Biosystems Engineering. 45:1719-1729
ISSN: 1615-7605
1615-7591
Popis: Polyhydroxybutyrate (PHB) is a bio-based, biodegradable and biocompatible plastic that has the potential to replace petroleum-based plastics. Lignocellulosic biomass is a promising feedstock for industrial fermentation to produce bioproducts such as polyhydroxybutyrate (PHB). However, the pretreatment processes of lignocellulosic biomass lead to the generation of toxic byproducts, such as furfural, 5-HMF, vanillin, and acetate, which affect microbial growth and productivity. In this study, to reduce furfural toxicity during PHB production from lignocellulosic hydrolysates, we genetically engineered Cupriavidus necator NCIMB 11599, by inserting the nicotine amide salvage pathway genes pncB and nadE to increase the NAD(P)H pool. We found that the expression of pncB was the most effective in improving tolerance to inhibitors, cell growth, PHB production and sugar consumption rate. In addition, the engineered strain harboring pncB showed higher PHB production using lignocellulosic hydrolysates than the wild-type strain. Therefore, the application of NAD salvage pathway genes improves the tolerance of Cupriavidus necator to lignocellulosic-derived inhibitors and should be used to optimize PHB production.
Databáze: OpenAIRE
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