Plasma levels of soluble ST2, but not IL-33, correlate with the severity of alcoholic liver disease

Autor: Zijian Sun, Wei Zhang, Qingsheng Liang, Lin Han, Lei Jin, Jun Zhao, Ji-yuan Zhang, Zhengsheng Zou, Ming Shi, Ang Huang, Binxia Chang, Shuli Hao, Hui Tian, Guangju Teng, Miaomiao Gao, Ying Sun
Rok vydání: 2018
Předmět:
Adult
Lipopolysaccharides
Male
0301 basic medicine
medicine.medical_specialty
Alcoholic liver disease
medicine.medical_treatment
Severity of Illness Index
Monocytes
Proinflammatory cytokine
End Stage Liver Disease
03 medical and health sciences
Liver disease
interleukin‐33
0302 clinical medicine
Liver Function Tests
Internal medicine
medicine
Humans
Lobules of liver
Liver Diseases
Alcoholic
Aged
sST2
Tumor Necrosis Factor-alpha
business.industry
Albumin
Original Articles
Cell Biology
Middle Aged
Interleukin-33
Prognosis
medicine.disease
Interleukin-1 Receptor-Like 1 Protein
030104 developmental biology
Endocrinology
Cytokine
Liver
Solubility
Case-Control Studies
030220 oncology & carcinogenesis
monocyte
Disease Progression
Molecular Medicine
Biomarker (medicine)
Original Article
Liver function
business
Biomarkers
alcoholic liver disease
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-1838
DOI: 10.1111/jcmm.13990
Popis: Alcoholic liver disease (ALD) is a complication that is a burden on global health and economy. Interleukin‐33 (IL‐33) is a newly identified member of the IL‐1 cytokine family and is released as an “alarmin” during inflammation. Soluble suppression of tumourigenicity 2 (sST2), an IL‐33 decoy receptor, has been reported as a new biomarker for the severity of systemic and highly inflammatory diseases. Here, we found the levels of plasma sST2, increased with the disease severity from mild to severe ALD. Importantly, the plasma sST2 levels in ALD patients not only correlated with scores for prognostic models (Maddrey's discriminant function, model for end‐stage liver disease and Child‐Pugh scores) and indexes for liver function (total bilirubin, international normalized ratio, albumin, and cholinesterase) but also correlated with neutrophil‐associated factors as well as some proinflammatory cytokines. In vitro, lipopolysaccharide‐activated monocytes down‐regulated transmembrane ST2 receptor but up‐regulated sST2 mRNA and protein expression and produced higher levels of tumour necrosis factor‐α (TNF‐α). By contrast, monocytes pretreated with recombinant sST2 showed decreased TNF‐α production. In addition, although plasma IL‐33 levels were comparable between healthy controls and ALD patients, we found the IL‐33 expression in liver tissues from ALD patients was down‐regulated at both RNA and protein levels. Immunohistochemical staining further showed that the decreased of IL‐33‐positive cells were mainly located in liver lobule area. These results suggested that sST2, but not IL‐33, is closely related to the severity of ALD. Consequently, sST2 could be used as a potential biomarker for predicting the prognosis of ALD.
Databáze: OpenAIRE
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