Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase
| Autor: | Narayanan Kasinathan, Subrahmanyam Mallikarjuna Volety, Venkata Rao Josyula |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Liposome
Chromatography Article Subject Carrier system Central composite design business.industry lcsh:RS1-441 Factorial experiment Biotechnology lcsh:Pharmacy and materia medica chemistry.chemical_compound chemistry Hyaluronidase Phosphatidylcholine Hyaluronic acid Zeta potential medicine business medicine.drug Research Article |
| Zdroj: | Journal of Drug Delivery, Vol 2014 (2014) Journal of Drug Delivery |
| ISSN: | 2090-3022 2090-3014 |
| Popis: | Hyaluronidase is an enzyme that catalyzes breakdown of hyaluronic acid. This property is utilized for hypodermoclysis and for treating extravasation injury. Hyaluronidase is further studied for possible application as an adjuvant for increasing the efficacy of other drugs. Development of suitable carrier system for hyaluronidase would help in coadministration of other drugs. In the present study, the hyaluronidase was encapsulated in liposomes. The effect of variables, namely, phosphatidylcholine (PC), cholesterol, temperature during film formation (T1), and speed of rotation of the flask during film formation (SPR) on percentage of protein encapsulation, was first analyzed using factorial design. The study showed that level of phosphatidylcholine had the maximum effect on the outcome. The effect of interaction of PC and SPR required for preparation of nanoliposomes was identified by central composite design (CCD). The dependent variables were percentage protein encapsulation, particle size, and zeta potential. The study showed that ideal conditions for production of hyaluronidase loaded nanoliposomes are PC—140 mg and cholesterol 1/5th of PC when the SPR is 150 rpm andT1is 50°C. |
| Databáze: | OpenAIRE |
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