Pericellular oxygen monitoring with integrated sensor chips for reproducible cell culture experiments

Autor:	Jochen Kieninger, Joe Alexander Sandvik, Erik O. Pettersen, Kuppusamy Aravindalochanan, Gerald Urban
Rok vydání:	2014
Předmět:	Cell Respiration Cell Culture Techniques chemistry.chemical_element Breast Neoplasms Biosensing Techniques Oxygen Oxygen Consumption Cell Line Tumor Materials Testing Respiration Humans Brain Neoplasms Reproducibility of Results Hypoxia (environmental) Equipment Design Original Articles Cell Biology General Medicine Cell Hypoxia Oxygen tension Electrochemical gas sensor chemistry Cell culture MCF-7 Cells Biophysics Female Limiting oxygen concentration Oxygen sensor
Zdroj:	Cell Proliferation Cell Proliferation; Vol 47
ISSN:	0960-7722
DOI:	10.1111/j.1365-2184.2013.12089.x
Popis:	OBJECTIVES: Here we present an application, in two tumour cell lines, based on the Sensing Cell Culture Flask system as a cell culture monitoring tool for pericellular oxygen sensing. MATERIALS AND METHODS: T‐47D (human breast cancer) and T98G (human brain cancer) cells were cultured either in atmospheric air or in a glove‐box set at 4% oxygen, in both cases with 5% CO(2) in the gas phase. Pericellular oxygen tension was measured with the help of an integrated sensor chip comprising oxygen sensor arrays. RESULTS: Obtained results illustrate variation of pericellular oxygen tension in attached cells covered by stagnant medium. Independent of incubation conditions, low pericellular oxygen concentration levels, usually associated with hypoxia, were found in dense cell cultures. CONCLUSIONS: Respiration alone brought pericellular oxygen concentration down to levels which could activate hypoxia‐sensing regulatory processes in cultures believed to be aerobic. Cells in culture believed to experience conditions of mild hypoxia may, in reality, experience severe hypoxia. This would lead to incorrect assumptions and suggests that pericellular oxygen concentration readings are of great importance to obtain reproducible results when dealing with hypoxic and normoxic (aerobic) incubation conditions. The Sensing Cell Culture Flask system allows continuous monitoring of pericellular oxygen concentration with outstanding long‐term stability and no need for recalibration during cell culture experiments. The sensor is integrated into the flask bottom, thus in direct contact with attached cells. No additional equipment needs to be inserted into the flask during culturing. Transparency of the electrochemical sensor chip allows optical inspection of cells attached on top of the sensor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::562ada15d13916dddf212bd9bfb50d40 https://doi.org/10.1111/j.1365-2184.2013.12089.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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