Topographical organization of mammillary neurogenesis and efferent projections in the mouse brain
| Autor: | Hongzhi Liu, Jinyun Wu, Ling Gong, Yongjie Hou, Yun Shi, Zhengang Yang, Mengmeng Shao, Jiangteng Lu, Zhuhao Wu, Qi Zhang, Yanqing Qi, Miao He |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Mammillary Bodies Mammillary body Neurogenesis Efferent Neuronal differentiation inducible genetic fate mapping topographic principles Nerve Tissue Proteins Biology Axonal tracing Spatial memory General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine projection mapping Animals mammillary body lcsh:QH301-705.5 Mice Knockout Neurons intersectional targeting Normal anatomy Fezf2 Cell Differentiation DNA-Binding Proteins 030104 developmental biology lcsh:Biology (General) Knockout mouse Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 34, Iss 6, Pp 108712-(2021) |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2021.108712 |
| Popis: | Summary: The mammillary body is a hypothalamic nucleus that has important functions in memory and spatial navigation, but its developmental principles remain not well understood. Here, we identify progenitor-specific Fezf2 expression in the developing mammillary body and develop an intersectional fate-mapping approach to demonstrate that Fezf2+ mammillary progenitors generate mammillary neurons in a rostral-dorsal-lateral to caudal-ventral-medial fashion. Axonal tracing from different temporal cohorts of labeled mammillary neurons reveal their topographical organization. Unsupervised hierarchical clustering based on intrinsic properties further identify two distinct neuronal clusters independent of birthdates in the medial nuclei. In addition, we generate Fezf2 knockout mice and observe the smaller mammillary body with largely normal anatomy and mildly affected cellular electrophysiology, in contrast to more severe deficits in neuronal differentiation and projection in many other brain regions. These results indicate that Fezf2 may function differently in the mammillary body. Our results provide important insights for mammillary development and connectivity. |
| Databáze: | OpenAIRE |
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