Clonal lymphocytes are detectable in only some cases of MDS
| Autor: | Alice K. Jacobs, J. A. Whittaker, D. J. Culligan, P. G. Cachia, Rose Ann Padua |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Male X Chromosome Genetic Linkage Lymphocyte Population Molecular Sequence Data Biology Polymerase Chain Reaction law.invention law hemic and lymphatic diseases medicine Humans Lymphocytes education Polymerase chain reaction Aged Aged 80 and over Gene Rearrangement education.field_of_study Base Sequence Lymphoma Non-Hodgkin Hematology DNA Middle Aged Leukemia Lymphocytic Chronic B-Cell Hypervariable region Clone Cells Blotting Southern medicine.anatomical_structure Polyclonal antibodies Myelodysplastic Syndromes Immunology Monoclonal biology.protein Immunoglobulin heavy chain Female Clone (B-cell biology) Immunoglobulin Heavy Chains |
| Zdroj: | British journal of haematology. 81(3) |
| ISSN: | 0007-1048 |
| Popis: | Clonal analysis of lymphocytes from patients with myelodysplastic syndrome (MDS) has been carried out using X-chromosome inactivation patterns detected by the probe M27 beta, and by polymerase chain reaction amplification of the immunoglobulin heavy chain gene hypervariable region, CDR3. Of 32 female patients heterozygous for M27 beta only seven (22%) demonstrate monoclonality of peripheral blood lymphocytes. 12 (37%) give unequivocal polyclonal results and the remaining cases give patterns of X-inactivation which cannot be interpreted either way. A study of 68 MDS patients showed five (7%) with a population of B-cells with a monoclonal rearrangement of CDR3 compared with none out of 60 normal individuals, none out of 15 with B-non Hodgkin lymphoma (B-NHL) in remission and 19 out of 25 (75%) of cases of B-chronic lymphocytic leukaemia (B-CLL). Monoclonal lymphocytes were found by both techniques in only two females with MDS. We conclude that the presence of polyclonal lymphocytes is a common finding in patients with MDS. |
| Databáze: | OpenAIRE |
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