Sulforaphane Increase Mitochondrial Biogenesis-Related Gene Expression in the Hippocampus and Suppresses Age-Related Cognitive Decline in Mice
| Autor: | Sunao Shimizu, Shuya Kasai, Hiromi Yamazaki, Yota Tatara, Junsei Mimura, Máté János Engler, Kunikazu Tanji, Yoshikazu Nikaido, Takuro Inoue, Hiroyuki Suganuma, Koichi Wakabayashi, Ken Itoh |
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| Rok vydání: | 2022 |
| Předmět: |
NRF2
sulforaphane glucoraphanin cognitive decline PGC1α TFAM mitochondrial biogenesis Aging Organelle Biogenesis Organic Chemistry Gene Expression General Medicine DNA Hippocampus Catalysis Computer Science Applications Inorganic Chemistry Mice Isothiocyanates Sulfoxides Animals Cognitive Dysfunction Physical and Theoretical Chemistry Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 15; Pages: 8433 |
| ISSN: | 1422-0067 |
| Popis: | Sulforaphane (SFN) is a potent activator of the transcriptional factor, Nuclear Factor Erythroid 2 (NF-E2)-Related factor 2 (NRF2). SFN and its precursor, glucoraphanin (sulforaphane glucosinolate, SGS), have been shown to ameliorate cognitive function in clinical trials and in vivo studies. However, the effects of SGS on age-related cognitive decline in Senescence-Accelerated Mouse Prone 8 (SAMP8) is unknown. In this study, we determined the preventive potential of SGS on age-related cognitive decline. One-month old SAMP8 mice or control SAM resistance 1 (SAMR1) mice were fed an ad libitum diet with or without SGS-containing broccoli sprout powder (0.3% w/w SGS in diet) until 13 months of age. SGS significantly improved long-term memory in SAMP8 at 12 months of age. Interestingly, SGS increased hippocampal mRNA and protein levels of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC1α) and mitochondrial transcription factor A (TFAM), which are master regulators of mitochondrial biogenesis, both in SAMR1 and SAMP8 at 13 months of age. Furthermore, mRNAs for nuclear respiratory factor-1 (NRF-1) and mitochondrial DNA-encoded respiratory complex enzymes, but not mitochondrial DNA itself, were increased by SGS in SAMP8 mice. These results suggest that SGS prevents age-related cognitive decline by maintaining mitochondrial function in senescence-accelerated mice. |
| Databáze: | OpenAIRE |
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