Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria
| Autor: | José Mauro Granjeiro, Victor R Martinez-Zelaya, Suelen Cristina Sartoretto, Mônica Diuana Calasans-Maia, José de Albuquerque Calasans-Maia, Rodrigo Figueiredo de Brito Resende, Rafael Seabra Louro, Carlos Fernando de Almeida Barros Mourão, Alexandre Malta Rossi, Adriana Terezinha Neves Novellino Alves, Thiago Schneider Werner Vianna |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
animal structures
Materials science lcsh:Biotechnology Biomedical Engineering Connective tissue Calvaria 02 engineering and technology Bone healing Bone morphogenetic protein Bone tissue engineering Article Microsphere Biomaterials Extracellular matrix 03 medical and health sciences 0302 clinical medicine bone regeneration lcsh:TP248.13-248.65 medicine critical-size defect Bone regeneration nanostructured carbonated hydroxyapatite lcsh:R5-920 030206 dentistry 021001 nanoscience & nanotechnology medicine.anatomical_structure embryonic structures 0210 nano-technology lcsh:Medicine (General) rhBMP-2 Biomedical engineering |
| Zdroj: | Journal of Functional Biomaterials, Vol 11, Iss 87, p 87 (2020) Journal of Functional Biomaterials Volume 11 Issue 4 |
| ISSN: | 2079-4983 |
| Popis: | Many biomaterials are used for Bone Morphogenetic Proteins (BMPs) delivery in bone tissue engineering. The BMP carrier system&rsquo s primary function is to hold these growth factors at the wound&rsquo s site for a prolonged time and provide initial support for cells to attach and elaborate the extracellular matrix for bone regeneration. This study aimed to evaluate the nanostructured carbonated hydroxyapatite microspheres (nCHA) as an rhBMP-2 carrier on rats calvaria. A total of fifteen male Wistar rats were randomly divided into three groups (n = 5): clot (control group), rhBMP-2 associated with collagen membrane (COL/rhBMP-2) or associated with the microspheres (nCHA/rhBMP-2). After 45 days, the calvaria defect samples were evaluated through histological, histomorphometric, and SR-µ CT analyses to investigate new-formed bone and connective tissue volume densities. The descriptive histological analysis showed that nCHA/rhBMP-2 improved bone formation compared to other groups. These results were confirmed by histomorphometric and SR-µ CT analysis that showed substantially defect area filling with a higher percentage of newly formed (36.24 ± 6.68) bone than those with the COL/rhBMP-2 (0.42 ± 0.40) and Clot (3.84 ± 4.57) (p < 0.05). The results showed that nCHA is an effective carrier for rhBMP-2 encouraging bone healing and an efficient alternative to collagen membrane for rhBMP-2 delivery. |
| Databáze: | OpenAIRE |
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