Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum
| Autor: | Andreas Pulfer, Paul Jenoe, Stuart A. Ralph, Igor Niederwieser, Nicolas M. B. Brancucci, Kathrin Witmer, Sophie Clara Oehring, Olivier Dietz, Suzette Moes, Till S. Voss, Johanna Wetzel, Christian Flueck, Pascal Maeser, Chaitali Dekiwadia, Ben J. Woodcroft |
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| Rok vydání: | 2012 |
| Předmět: |
Proteomics
Erythrocytes Bioinformatics Nuclear pore Plasmodium falciparum Protozoan Proteins Computational biology Transfection Mass Spectrometry Nucleus 03 medical and health sciences parasitic diseases Organelle Antigenic variation medicine Humans Nuclear protein 030304 developmental biology Cell Nucleus Genetics 0303 health sciences biology Research 030302 biochemistry & molecular biology Nuclear Proteins Nucleolus biology.organism_classification medicine.disease Malaria 3. Good health Cell nucleus medicine.anatomical_structure Proteome Transcription Chromatography Liquid IFA |
| Zdroj: | Genome Biology |
| ISSN: | 1465-6906 |
| Popis: | Background: The post genomic era of malaria research provided unprecedented insights into the biology of Plasmodium parasites. Due to the large evolutionary distance to model eukaryotes however we lack a profound understanding of many processes in Plasmodium biology. One example is the cell nucleus which controls the parasite genome in a development and cell cycle specific manner through mostly unknown mechanisms. To study this important organelle in detail we conducted an integrative analysis of the P. falciparum nuclear proteome. Results: We combined high accuracy mass spectrometry and bioinformatic approaches to present for the first time an experimentally determined core nuclear proteome for P. falciparum. Besides a large number of factors implicated in known nuclear processes one third of all detected proteins carry no functional annotation including many phylum or genus specific factors. Importantly extensive experimental validation using 30 transgenic cell lines confirmed the high specificity of this inventory and revealed distinct nuclear localization patterns of hitherto uncharacterized proteins. Further our detailed analysis of the core nuclear proteome identified novel protein domains potentially implicated in gene transcription pathways and sheds important new light on nuclear compartments and processes including regulatory complexes the nucleolus nuclear pores and nuclear import pathways. Conclusion: Our study provides comprehensive new insight into the biology of the Plasmodium nucleus and will serve as an important platform for dissecting general and parasite specific nuclear processes in malaria parasites. Moreover as the first nuclear proteome characterized in any protist organism it will provide an important resource for studying evolutionary aspects of nuclear biology. |
| Databáze: | OpenAIRE |
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