Effects of bee venom on the maturation of murine dendritic cells stimulated by LPS
| Autor: | Jongsoo Lee, Sung-Soo Kim, Han-Sung Lee, Mi-Yeon Song, Woo-Jin Shim, Ae-Ri Han, Seok-Hee Chung, Hyun-Dae Shin |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Chemokine Ratón medicine.medical_treatment Bone Marrow Cells Biology Mice Antigens CD Immunopathology Drug Discovery medicine Animals Pharmacology Autoimmune disease Chemokine CCL21 Dose-Response Relationship Drug Granulocyte-Macrophage Colony-Stimulating Factor Cell Differentiation Dendritic Cells Immunotherapy Dendritic cell medicine.disease Medicine Korean Traditional Up-Regulation Mice Inbred C57BL Bee Venoms medicine.anatomical_structure Immunology biology.protein Bone marrow Inflammation Mediators Immunosuppressive Agents CCL21 |
| Zdroj: | Journal of Ethnopharmacology. 120:215-219 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2008.08.014 |
| Popis: | Aim of study This study was performed to elicit the effectiveness of bee venom (BV), a traditional immunosuppressive Korean acupuncture agent, on the maturation of dendrtic cells (DCs). Materials and methods Immature dendritic cells (iDCs) were generated from mouse bone marrow cells with GM-CSF. After 10 days of initial differentiation, DCs were activated with lipopolysaccharides (LPS) for another 48h in the presence or absence of BV. Surface molecule analysis, intracytoplasmic staining of cytokines, FITC-conjugated antigen uptake, and transwell migration assays were conducted with iDCs and activated DCs. Results Up-regulation of costimulatory molecules, typical of mature DCs (mDCs) was inhibited by addition of BV. Pro-inflammatory cytokines were also found to be reduced with BV treatment in LPS-stimulated DC. A decrease in antigen uptake upon the maturation of DC was reversed in low dose BV treated mDC. In addition, BV treated mDC demonstrated reduced directional migration in response to CCL21, a lymphoid chemokine which directs mDC. Conclusions BV may have a therapeutic effect an on abnormally activated immune status, such as autoimmune rheumatoid arthritis, through an immune-modulatory effect on DC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect