Stimulation of Human Endothelium with IL-3 Induces Selective Basophil Accumulation In Vitro
| Autor: | Konstantinos Konstantopoulos, Monica M. Burdick, Sherry A. Hudson, Fatimah Bte Mustafa, Lina H. K. Lim, Bruce S. Bochner |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Integrins
Endothelium Neutrophils medicine.medical_treatment Immunology Integrin CCR3 chemical and pharmacologic phenomena Inflammation Biology Basophil Antibodies Umbilical Cord Chemokine receptor parasitic diseases Cell Adhesion medicine Humans Immunology and Allergy Cells Cultured hemic and immune systems Eosinophil Receptors Interleukin-3 Basophils Cell biology Eosinophils medicine.anatomical_structure Cytokine Selectins biology.protein Interleukin-3 Receptors Chemokine medicine.symptom |
| Zdroj: | The Journal of Immunology. 176:5346-5353 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.176.9.5346 |
| Popis: | Basophils have been shown to accumulate in allergic airways and other extravascular sites. Mechanisms responsible for the selective recruitment of basophils from the blood into tissue sites remain poorly characterized. In this study, we characterized human basophil rolling and adhesion on HUVECs under physiological shear flow conditions. Interestingly, treatment of endothelial cells with the basophil-specific cytokine IL-3 (0.01–10 ng/ml) promoted basophil and eosinophil, but not neutrophil, rolling and exclusively promoted basophil adhesion. Preincubation of HUVECs with an IL-3R-blocking Ab (CD123) before the addition of IL-3 inhibited basophil rolling and adhesion, implicating IL-3R activation on endothelial cells. Incubation of basophils with neuraminidase completely abolished both rolling and adhesion, indicating the involvement of sialylated structures in the process. Abs to the β1 integrins, CD49d and CD49e, as well as to P-selectin and P-selectin glycoprotein ligand 1, inhibited basophil rolling and adhesion. Furthermore, blocking chemokine receptors expressed by basophils, such as CCR2, CCR3, and CCR7, demonstrated that CCR7 was involved in the observed recruitment of basophils. These data provide novel insights into how IL-3, acting directly on endothelium, can cause basophils to preferentially interact with blood vessels under physiological flow conditions and be selectively recruited to sites of inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|