MORPHOFUNCTIONAL CHARACTERISTICS OF FIBROBLASTS (MCCOY CELL LINE) CULTURED WITH MAGNESIUM PREPARATIONS
| Autor: | A. V. Timofeeva, E. A. Ulubieva, L. V. Didenko, T. G. Borovaya, E. A. Kost, A. G. Avtandilov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
lcsh:Diseases of the circulatory (Cardiovascular) system
пиридоксин Cell chemistry.chemical_element magnesium orotate RM1-950 Extracellular matrix magnesium sulfate fibroblasts medicine Diseases of the circulatory (Cardiovascular) system Pharmacology (medical) оротат магния Fibroblast Magnesium ion pyridoxine сульфат магния biology electron microscopy business.industry Magnesium lcsh:RM1-950 Pyridoxine lcsh:Therapeutics. Pharmacology medicine.anatomical_structure chemistry Biochemistry lcsh:RC666-701 Cell culture RC666-701 biology.protein фибробласты Therapeutics. Pharmacology электронная микроскопия Cardiology and Cardiovascular Medicine business Elastin medicine.drug |
| Zdroj: | Racionalʹnaâ Farmakoterapiâ v Kardiologii, Vol 11, Iss 2, Pp 170-176 (2015) |
| ISSN: | 2225-3653 1819-6446 |
| Popis: | Aim. To study the effect of magnesium orotate, magnesium/pyridoxine combination and magnesium sulfate on fibroblast morphofunctional characteristics in cell culture of fibroblasts (McCoy line).Material and methods. The study of fibroblasts (McCoy line) with the addition of magnesium-containing preparations (magnesium orotate, magnesium/pyridoxine combination, magnesium sulphate) to the culture medium was performed using scanning and transmission electron microscopy.Results. When adding into the media magnesium orotate or magnesium/pyridoxine combination, significant changes in morphofunctional state of fibroblasts were noted, which were absent when adding magnesium sulfate. At that fibroblasts significantly promoted synthetic and secretory activity. This was expressed in the formation of amorphous and fibrous components in well-formed cell layers.Conclusion. Stimulation by magnesium ions of the proliferative activity of fibroblasts is shown in the morphological analysis of the effect of magnesium orotate or magnesium in combination with pyridoxine and magnesium sulfate on morphological and functional organization of fibroblasts. Revealed the predominant influence of magnesium orotate, and a less pronounced effect of magnesium in combination with pyridoxine on biosynthetic processes in the cells, including the synthesis of amorphous and fibrous components (protocollagen and elastin) of the extracellular matrix founded. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|