Therapeutic effect of gold nanoparticles on DSS-induced ulcerative colitis in mice with reference to interleukin-17 expression
| Autor: | Fadia K. Abdo, Amira M. Abdelmegid, Asmaa A. A. Kattaia, Fayza E. Ahmed |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Colon Science Anti-Inflammatory Agents Metal Nanoparticles Article 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine medicine Animals Gastrointestinal models Colitis Mice Inbred BALB C Multidisciplinary Therapeutic effect Dextran Sulfate Interleukin-17 medicine.disease Malondialdehyde Ulcerative colitis digestive system diseases Experimental models of disease Disease Models Animal 030104 developmental biology Dextran chemistry Colloidal gold Immunohistochemistry Medicine Colitis Ulcerative Female Interleukin 17 Gold 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Ulcerative colitis (UC) is among the most challenging human diseases. Nanotechnology has incontestable promising outcomes in inflammatory bowel diseases. This study aimed to investigate the therapeutic effect of naked gold nanoparticles (AuNPs) on dextran sodium sulphate (DSS) induced ulcerative colitis in mice. We also examined the expression of interleukin-17 (IL-17) following AuNPs treatment. Mice were randomly divided into control, DSS and DSS+ AuNPs groups. Severity of colitis was assessed by disease activity index (DAI) measurement. At the end of the experiment, the final body weights were recorded. The colon was dissected and processed for histopathological examinations by light and electron microscopes. Colon homogenates were prepared for assay of tissue malondialdehyde (MDA) and real-time PCR analysis of IL-17A. Immunohistochemical localization of IL-17A was carried out. Scanning electron microscopy (SEM) and Energy Dispersive X-ray (EDX) detector were used to detect the presence of AuNPs in the colonic tissue of DSS+ AuNPs groups. Our results showed that AuNPs effectively targeted the colonic tissue, and reduced changes induced by DSS. The underlying mechanisms could be related to anti-oxidant effect (as evident by decreasing tissue MDA) and anti-inflammatory potential of AuNPs. Our study draws attention to as a novel therapeutic strategy for treating UC. |
| Databáze: | OpenAIRE |
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