Imaging with99mTc ECDG Targeted at the Multifunctional Glucose Transport System: Feasibility Study with Rodents
| Autor: | David J. Yang, Donald A. Podoloff, Jong Jin Won, Ali Azhdarinia, Chang Guhn Kim, Chang Sok Oh, Dong Fang Yu, Naomi R. Schechter, Jerry Bryant, E. Edmund Kim |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Blood Glucose
Pathology medicine.medical_specialty Biodistribution Lung Neoplasms Ratón Scintigraphy Mice chemistry.chemical_compound Fluorodeoxyglucose F18 Glucosamine In vivo medicine Animals Insulin Tissue Distribution Radiology Nuclear Medicine and imaging Cysteine Radionuclide Imaging Fluorodeoxyglucose medicine.diagnostic_test business.industry Glucose transporter Organotechnetium Compounds Rats chemistry Feasibility Studies Radiopharmaceuticals business Technetium-99m medicine.drug |
| Zdroj: | Radiology. 226:465-473 |
| ISSN: | 1527-1315 0033-8419 |
| DOI: | 10.1148/radiol.2262011811 |
| Popis: | To evaluate the feasibility of technetium 99m ((99m)Tc) ethylenedicysteine-deoxyglucose (ECDG) imaging in tumor-bearing rodents.ECDG was synthesized by means of reacting ethylenedicysteine with glucosamine, with carbodiimide as the coupling agent. Hexokinase assays were performed at an ultraviolet wavelength of 340 nm. To determine whether blood glucose level could be altered, ECDG or glucosamine was injected into six rats. In a separate study, ECDG followed by insulin was administered to three rats. To determine biodistribution, lung tumor cells were intramuscularly injected into the hind legs of 18 nude mice. The animals were then injected with (99m)Tc ECDG or fluorine 18 ((18)F) fluorodeoxyglucose (FDG) (0.037-0.074 MBq per mouse). Radioactivity was measured in tissue excised from the animals. Scintigraphy was performed in three groups: in group 1 to demonstrate that different-sized tumors could be imaged after (99m)Tc ECDG administration, in group 2 to ascertain whether tumor uptake of (99m)Tc ECDG was perfusion related, and in group 3 to demonstrate that tumor uptake of (99m)Tc ECDG occurred by means of a glucose-mediated process.ECDG was positive for phosphorylation at hexokinase assay. Blood glucose level increased with ECDG injection and decreased with insulin administration. Tumor-to-brain tissue and tumor-to-muscle tissue ratios of (99m)Tc ECDG uptake were higher than those of (18)F FDG uptake. Scintigraphic results demonstrated the feasibility of (99m)Tc ECDG imaging.There are similarities between (99m)Tc ECDG uptake and (18)F FDG uptake in tumors, and study findings supported the potential use of (99m)Tc ECDG as a functional imaging agent. |
| Databáze: | OpenAIRE |
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