Novel insights about the ascidian dopamine system: Pharmacology and phylogenetics of catecholaminergic receptors on the Phallusia nigra immune cells
| Autor: | Rodrigo Nunes-da-Fonseca, Andressa de Abreu Mello, Taynan Motta Portal, Jessica Fernandes de Souza, Cintia Monteiro de Barros |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Phallusia Adrenergic receptor Dopamine Aquatic Science Biology Pharmacology 03 medical and health sciences Immune system medicine Animals Environmental Chemistry Urochordata Receptor Phylogeny Catecholaminergic Neurotransmitter Agents Receptors Dopamine D2 Dopaminergic 04 agricultural and veterinary sciences General Medicine biology.organism_classification 030104 developmental biology Dopamine receptor 040102 fisheries 0401 agriculture forestry and fisheries Signal Transduction medicine.drug |
| Zdroj: | Fish & Shellfish Immunology. 109:41-50 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2020.11.022 |
| Popis: | Dopamine (DA) is an important molecule that plays a role in the nervous and immune systems. DA is produced by a wide variety of animals and it is considered one of the oldest neurotransmitters. However, its specific function in immune cells has not been completely revealed. In a group of chordate animals, the ascidians, DA is reported to be produced by cells in the central nervous system (CNS); however, no dopaminergic receptor in their genomes has been described until now. Because this is an integrating characteristic of the ascidian dopamine system, here it was investigated the pharmacology, function, and phylogeny of DA and dopaminergic receptors (DRs) in the modulation of nitric oxide (NO) in the Phallusia nigra immune cells. The data disclosed, for the first time, that DA modulates NO production by immune cells. Its modulation probably occurs though adrenergic receptors, which display a special characteristic, in that they are capable of binding to noradrenaline (NA) and DA. A pharmacological analysis revealed that receptors present on the ascidian immune cells showed a high affinity to butaclamol, a non-selective D2-class receptor, increasing NO production. In addition, calcium intracellular mobilization was observed when DA was added to immune cells. In conclusion, the data revealed novel insights about the presence of catecholaminergic receptors (CRs) on the P. nigra immune cells, indicating that ascidian CRs have special pharmacological characteristics that are worth highlighting from an evolutionary point of view. |
| Databáze: | OpenAIRE |
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