Plasticity of IL-2 and IL-2 receptor chains in rat lymphoid tissues in situ after stimulation with staphylococcal enterotoxin A
| Autor: | Richard Fairless, Eberhard Weihe, Michael Bette, Tobias Geisler, Martin K.-H. Schäfer, Horacio Romeo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Interferon Inducers
Lymphoid Tissue T cell T-Lymphocytes Immunology Spleen Biology Biochemistry Enterotoxins Mice Downregulation and upregulation medicine Superantigen Immunology and Allergy Animals IL-2 receptor RNA Messenger Receptor Molecular Biology Receptors Interleukin-2 Hematology Molecular biology Rats medicine.anatomical_structure Lymphatic system Gene Expression Regulation Autoradiography Interleukin-2 CD8 |
| Zdroj: | Cytokine. 22(6) |
| ISSN: | 1043-4666 |
| Popis: | Although the effects of mitogens on the synthesis of interleukin-2 (IL-2) and IL-2 receptor (IL-2r) have been described, a detailed in situ analysis of the spatio-temporal changes of the expression of the IL-2 gene and the three IL-2r components in lymphoid tissues is still missing. Therefore, we analyzed the IL-2 and IL-2r expression after a staphylococcal enterotoxin A (SEA)-induced T cell activation on a cellular and anatomical basis in the Wistar rat. SEA caused a rapid induction of IL-2 mRNA in T cells of spleen, lymph node, and thymus, followed by the appearance of high systemic IL-2 serum levels (5 ng/ml), and a significant increase of CD25 on CD4+ and CD8+ lymphocytes. The histotopographic analysis of the IL-2r chains revealed a strong upregulation of IL-2r alpha (alpha) and IL-2r beta (beta) mRNAs in similar T cell specific compartments of spleen, lymph node, and thymus as seen for IL-2 mRNA. The abundant constitutive expression of IL-2r gamma (gamma) mRNA was unaffected by SEA. The parallel upregulation of IL-2, IL-2ralpha, and beta chains in conjunction with the continuous presence of the IL-2rgamma chain predominantly in T cell regions of immune organs suggests that the biological effects of IL-2 are essentially limited to T cells, at least after superantigen stimulation. |
| Databáze: | OpenAIRE |
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