Relationship Between p73 Polymorphism and the Immunohistochemical Profile of the Full-length (TAp73) and NH2-truncated (ΔNp73) Isoforms in Tunisian Patients
| Autor: | Sabeh Mzabi Regaya, Amine Lounis Mohamed, Taher Khalfallah, Abdelmajid Ben Hmida, Saadia Bouraoui, Ines Chaar, Lilia Kriaa Ben Mahmoud, Amira Toumi Arfaoui, Mariem Khiari |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Tunisia Histology Colorectal cancer Loss of Heterozygosity Kaplan-Meier Estimate Biology Gastroenterology Pathology and Forensic Medicine Metastasis Loss of heterozygosity Risk Factors Internal medicine Genotype medicine Humans Protein Isoforms Intestinal Mucosa Allele Genetic Association Studies Retrospective Studies Tumor Suppressor Proteins Case-control study Nuclear Proteins Tumor Protein p73 Heterozygote advantage Prognosis medicine.disease Immunohistochemistry Primary tumor DNA-Binding Proteins Medical Laboratory Technology Case-Control Studies Female Colorectal Neoplasms Polymorphism Restriction Fragment Length |
| Zdroj: | Applied Immunohistochemistry & Molecular Morphology. 18:546-554 |
| ISSN: | 1541-2016 |
| Popis: | Background We examined the association of one linked GC/AT polymorphism at p73 with the risk of colorectal cancer. Aim In this study, we investigated whether this polymorphism was related to the risk of colorectal cancer, and whether there were relationships between the polymorphism and loss of heterozygosity, protein expression, or clinicopathologic variables. Materials and methods The p73 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 150 Tunisian patients with colorectal cancer and in 204 healthy control subjects. Immunohistochemistry was performed on normal mucosa, primary tumor, and metastasis. Results The frequencies of the genotypes were 52% for wild-type (GC/GC), 31% for heterozygotes (GC/AT), and 17% for variants(AT/AT) in patients, and 54%, 35%, and 11% in controls, respectively. There were no significant differences of the frequencies of the 3 genotypes between the patients and controls (P=0.11). We did not find any relationship of the genotypes with clinicopathologic features of patients. We found that patients with the GC/GC genotype had a significantly more favorable clinical outcome than the patients with the AT variants (AT/AT or GC/AT genotype). There were no significant difference between tumoral immunostaining and p73 polymorphism (P=0.16) but we found that the samples carrying the AT allele showed a tendency to be more stained in tumor. No loss of heterozygosity was observed at p73 locus. Our results suggest that the AT/AT genotype is significantly associated with poor prognosis in colorectal cancer. All these findings suggest that p73 polymorphism analysis may provide useful prognostic information for colorectal cancer patients. |
| Databáze: | OpenAIRE |
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