Alteration of connexin expression is an early signal for chronic kidney disease
| Autor: | Jean-Claude Dussaule, Christos E. Chadjichristos, Julie Toubas, Mouna Mael-ainin, Anne-Cecile Huby, Samantha Beck, Anne-Laure Pageaud, Christos Chatziantoniou |
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| Přispěvatelé: | Remodelage et Reparation du Tissu Renal, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Maladies rénales fréquentes et rares : des mécanismes moléculaires à la médecine personnalisée (CoRaKID), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) |
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Anti-Glomerular Basement Membrane Disease Physiology Urinary system Fluorescent Antibody Technique Connexin Blood Pressure Inflammation urologic and male genital diseases Connexins Blood Urea Nitrogen Mice Fibrosis Renin Renin–angiotensin system Animals Medicine Chemokine CCL2 Mice Knockout Reverse Transcriptase Polymerase Chain Reaction business.industry Editorial Focus medicine.disease Immunohistochemistry Angiotensin II Proteinuria Liver Connexin 43 Chronic Disease Immunology RNA Kidney Diseases medicine.symptom business Cell Adhesion Molecules [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Biomarkers Kidney disease |
| Zdroj: | American Journal of Physiology. Renal Physiology American Journal of Physiology. Renal Physiology, 2011, 301 (1), pp.F24-F32. ⟨10.1152/ajprenal.00255.2010⟩ |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00255.2010 |
| Popis: | International audience; Chronic kidney disease is promoted by a variety of factors that induce chronic inflammation and fibrosis. Inflammation and excessive scaring have been recently associated with disruptions of the gap junction-mediated intercellular communication. Nevertheless, little is known about alterations of the expression of gap junction proteins such as connexin (Cx) 43 and 37 in chronic renal disease. In this study, we investigated the expression of these two Cxs in the hypertensive RenTg mice, the anti-glomerular basement membrane glomerulonephritis, and the unilateral ureteral obstruction models, all leading to the development of chronic kidney disease in mice. Expression of Cx43 was almost negligible in the renal cortex of control mice. In contrast, Cx43 was markedly increased in the endothelium of peritubular and glomerular capillaries of the 3-mo-old RenTg mice, in the glomeruli of mice suffering from glomerulonephritis, and in the tubules after obstructive nephropathy. The Cx43 expression pattern was paralleled closely by that of the adhesion markers such as vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 as well as the inflammatory biomarker monocyte chemoattractant protein-1. In contrast, Cx37 that was abundantly expressed in the renal cortex of healthy mice was markedly decreased in the three experimental models. Interestingly, Cx43+/− mice showed restricted expression of VCAM-1 after 2 wk of obstructive nephropathy. These findings suggest the importance of Cxs as markers of chronic renal disease and indicate that these proteins may participate in the inflammatory process during the development of this pathology. |
| Databáze: | OpenAIRE |
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