Biological Characteristics of Foam Cell Formation in Smooth Muscle Cells Derived from Bone Marrow Stem Cells
| Autor: | Cai-wen Duan, Zhengrong Mei, Chenglai Xia, Shi-huang Li, Peng-ke Yan |
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| Rok vydání: | 2011 |
| Předmět: |
Male
caveolin-1 Cell type Myocytes Smooth Muscle ABCA1 Bone Marrow Cells Biology Applied Microbiology and Biotechnology Muscle Smooth Vascular Rats Sprague-Dawley Extracellular matrix Bone marrow mesenchymal stem cells Downregulation and upregulation Myosin vascular smooth muscle cells Animals Scavenger receptor Molecular Biology Cells Cultured Ecology Evolution Behavior and Systematics Foam cell SR-A Reverse Transcriptase Polymerase Chain Reaction Bone Marrow Stem Cell Cell Biology Flow Cytometry musculoskeletal system Rats Cell biology Immunology cardiovascular system atherosclerosis Research Paper Foam Cells Developmental Biology Transforming growth factor |
| Zdroj: | International Journal of Biological Sciences |
| ISSN: | 1449-2288 |
| Popis: | Bone marrow mesenchymal stem cells (BMSC) can differentiate into diverse cell types, including adipogenic, osteogenic, chondrogenic and myogenic lineages. There are lots of BMSC accumulated in atherosclerosis vessels and differentiate into VSMC. However, it is unclear whether VSMC originated from BMSC (BMSC-SMC) could remodel the vessel in new tunica intima or promote the pathogenesis of atherosclerosis. In this study, BMSC were differentiated into VSMC in response to the transforming growth factor β (TGF-β) and shown to express a number of VSMC markers, such as α-smooth muscle actin (α-SMA) and smooth muscle myosin heavy chain1 (SM-MHC1). BMSC-SMC became foam cells after treatment with 80 mg/L ox-LDL for 72 hours. Ox-LDL could upregulate scavenger receptor class A (SR-A) but downregulate the ATP-binding cassette transporter A1 (ABCA1) and caveolin-1 protein expression, suggesting that modulating relative protein activity contributes to smooth muscle foam cell formation in BMSC-SMC. Furthermore, we found that BMSC-SMC have some biological characteristics that are similar to VSMC, such as the ability of proliferation and secretion of extracellular matrix, but, at the same time, retain some biological characteristics of BMSC, such as a high level of migration. These results suggest that BMSC-SMC could be induced to foam cells and be involved in the development of atherosclerosis. |
| Databáze: | OpenAIRE |
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