Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
| Autor: | Branka Vinterhalter, Ljiljana Tubić, Jovana Stanković-Jeremić, Slavica Ninković, Sofija Stupar, Jelena Savić, Aleksandar Cingel, Milan Dragićević, Vuk Maksimović, Nina Devrnja, Tatjana Ćosić, Vele Tešević |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Plant Science Biology French marigold 01 natural sciences Article French Marigold essential oil law.invention Transcriptome 03 medical and health sciences chemistry.chemical_compound law lcsh:Botany Bioassay Colorado potato beetle Food science Ecology Evolution Behavior and Systematics Essential oil 2. Zero hunger Ecology fungi food and beverages Fructose 15. Life on land biology.organism_classification lcsh:QK1-989 030104 developmental biology chemistry starch metabolism potato PEST analysis microarray 010606 plant biology & botany |
| Zdroj: | Plants, Vol 10, Iss 172, p 172 (2021) Plants Volume 10 Issue 1 |
| ISSN: | 2223-7747 |
| Popis: | Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change > 2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars&rsquo metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems. |
| Databáze: | OpenAIRE |
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