Antioxidant effect of Resveratrol: Change in MAPK cell signaling pathway during the aging process
| Autor: | Camila Amaro Caldeira, Filipe Nogueira Franco, Míriam Martins Chaves, Alessandra Aguiar Vieira, Milena Almeida Santos, Glaucy Rodrigues de Araújo, Raquel Cunha Lara |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Aging Health (social science) Antioxidant medicine.medical_treatment Resveratrol Pharmacology medicine.disease_cause Peripheral blood mononuclear cell Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Stilbenes medicine Humans 030212 general & internal medicine skin and connective tissue diseases Protein kinase B Aged chemistry.chemical_classification Reactive oxygen species 030214 geriatrics Hydrogen Peroxide Middle Aged Oxidative Stress chemistry Polyphenol Leukocytes Mononuclear Geriatrics and Gerontology Gerontology Oxidative stress Signal Transduction |
| Zdroj: | Archives of Gerontology and Geriatrics. 92:104266 |
| ISSN: | 0167-4943 |
| DOI: | 10.1016/j.archger.2020.104266 |
| Popis: | Aging is characterized by a progressive loss of physiological integrity. One common denominator is the increase of reactive oxygen species (ROS) caused by inhibition of important antioxidant pathways. Resveratrol is a polyphenol known for its potent antioxidant activity. However, antioxidant pathways activated by them change with aging. The objective of our study was to verify the antioxidant effect of resveratrol in an oxidative stress environment in Human Mononuclear Cells (PBMC) from donors with different ages. Resveratrol (5 μM), a stimulus with H2O2 (0,64 % v/v) in addition to inhibitors of PKA, AkT/PKB and MAPK signaling pathways were used in chemiluminescence assay. An incresed basal production of ROS was observed in the elderly than in the middle-aged group. Resveratrol was able to reduce ROS in both groups, but with greater efficiency in the middle-aged group. By inhibiting PKA, Akt/PKB and MAPK signaling pathways we observed that resveratrol presented an altered performance in the aging process, changing signaling pattern of MAPK pathway. |
| Databáze: | OpenAIRE |
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