ANG II-induced tyrosine phosphorylation stimulates phospholipase C-gamma 1 and Cl-channels in mesangial cells
| Autor: | Mario B. Marrero, He-Ping Ma, Brian N. Ling, Bernhard Schieffer, Kenneth E. Bernstein |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Physiology Inositol 1 4 5-Trisphosphate Rats Sprague-Dawley chemistry.chemical_compound Chloride Channels Internal medicine medicine Animals Phosphorylation Tyrosine Inositol phosphate chemistry.chemical_classification Phospholipase C Mesangial cell Phospholipase C gamma Chemistry Angiotensin II Tyrosine phosphorylation Intracellular Membranes Cell Biology Glomerular Mesangium Rats Cell biology Isoenzymes Endocrinology Type C Phospholipases Calcium Tyrosine kinase |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1996.270.6.c1834 |
| Popis: | Angiotensin II (ANG II)-induced, activation of phospholipase C (PLC) and Ca(2+)-dependent Cl-channels is an important signal transduction pathway for mesangial cell contraction and growth. Although ANG II receptors are traditionally though to be G protein coupled, recent evidence suggests that they may also mediate protein tyrosine phosphorylation. In cultured rat mesangial cells, 10(-7) MANG II stimulated the tyrosine phosphorylation of PLC-gamma 1 and elevation of intracellular inositol 1,4,5-trisphosphate (IP3) and Ca2+ levels; peak response occurred within 0.5 min. In cell-attached patches, ANG II stimulated the activity of Ca(2+)-dependent, 3- to 4-pS Cl-channels (number of channels x open probability) from 0.063 +/- 0.022 to 0.77 +/- 0.20. Tyrosine kinase inhibition with genistein or herbimycin A blocked all four ANG II-induced responses. We conclude the following. 1) Stimulation of inositol phosphate hydrolysis by PLC, release of IP3-dependent intracellular Ca2+ stores, and activation of Ca(2+)-dependent C1-channels by ANG II are dependent on the tyrosine phosphorylation of PLC-gamma 1.2) This ANG II-induced signal transduction cascade provides a possible mechanism for both the contractile and growth-stimulating effects of ANG II on glomerular mesangial cells. |
| Databáze: | OpenAIRE |
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