Cryo-laser scanning confocal microscopy of diffusible plant compounds
| Autor: | René Siret, Cédric Gaillard, Marc Lahaye, Kévin Vidot, Camille Rivard |
|---|---|
| Přispěvatelé: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Groupe de Recherche en Agroalimentaire sur les Produits et les Procédés (GRAPPE), Institut National de la Recherche Agronomique (INRA)-Ecole supérieure d'Agricultures d'Angers (ESA), Département Caractérisation et Elaboration des Produits Issus de l'Agriculture (CEPIA), RFI Food for Tomorrow-Cap Aliment, USC 1422 Groupe de Recherche en Agroalimentaire sur les Produits et les Procédés, Ecole Supérieure d'Agriculture (ESA)-Institut National de la Recherche Agronomique (INRA), Vidot, Kévin, Gaillard, Cedric, Lahaye, Marc |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cryogenic fixation [SDV]Life Sciences [q-bio] Grape Plant Science lcsh:Plant culture 01 natural sciences Cryo-confocal microscopy 03 medical and health sciences In vivo Genetics Confocal laser scanning microscopy lcsh:SB1-1110 Sample preparation lcsh:QH301-705.5 Large field of view Metallic ions Chemistry Methodology Apple food and beverages Cryogenic fixation Cryo-confocal microscopy Metallic ions Phenolics Apple Grape Plant tissue Fluorescence Staining 030104 developmental biology lcsh:Biology (General) Biophysics Phenolics 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Methods Plant Methods, BioMed Central, 2018, 14 (1), pp.1-8. ⟨10.1186/s13007-018-0356-x⟩ Plant Methods 1 (14), 1-8. (2018) Plant Methods, Vol 14, Iss 1, Pp 1-8 (2018) |
| ISSN: | 1746-4811 |
| DOI: | 10.1186/s13007-018-0356-x⟩ |
| Popis: | Background The in vivo observation of diffusible components, such as ions and small phenolic compounds, remains a challenge in turgid plant organs. The analytical techniques used to localize such components in water-rich tissue with a large field of view are lacking. It remains an issue to limit compound diffusion during sample preparation and observation processes. Results An experimental setup involving the infusion staining of plant tissue and the cryo-fixation and cryo-sectioning of tissue samples followed by fluorescence cryo-observation by laser scanning confocal microscopy (LSCM) was developed. This setup was successfully applied to investigate the structure of the apple fruit cortex and table grape berry and was shown to be relevant for localizing calcium, potassium and flavonoid compounds. Conclusion The cryo-approach was well adapted and opens new opportunities for imaging other diffusible components in hydrated tissues. |
| Databáze: | OpenAIRE |
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