Effect of prolactin on follicle-stimulating hormone receptor binding and progesterone production in cultured porcine granulosa cells
| Autor: | Misty Blanchette Porter, Cynthia K. Sites, John R. Brumsted |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
endocrine system
medicine.medical_specialty Swine medicine.drug_class Granulosa cell Biology Iodine Radioisotopes Internal medicine medicine Animals Ovarian follicle Cells Cultured Progesterone Granulosa Cells Sheep Estradiol Follicle-stimulating hormone receptor binding Obstetrics and Gynecology Drug Synergism Prolactin medicine.anatomical_structure Endocrinology Reproductive Medicine Hormone receptor Receptors FSH Female Folliculogenesis Follicle Stimulating Hormone Gonadotropin Follicle-stimulating hormone receptor hormones hormone substitutes and hormone antagonists |
| Zdroj: | Fertility and Sterility. 73:99-105 |
| ISSN: | 0015-0282 |
| Popis: | Objective: To determine the effect of prolactin (PRL) on follicle-stimulating hormone receptor (FSH-R) binding and progesterone (P) production in cultured porcine granulosa cells. Design: Controlled experiment. Setting: Academic research laboratory. Intervention(s): Immature granulosa cells were cultured in a serum-free medium. All cell populations were supplemented with porcine (p) FSH and cultured in the absence or presence of ovine (o) PRL. Main Outcome Measure(s): Specific pFSH-R binding and P in medium. Result(s): In the control cells, FSH-R binding increased sixfold and P production increased 700-fold by day 4. Physiologic levels of oPRL potentiated the action of pFSH and resulted in a further 50% increase in pFSH-R binding and P production by day 4 over that in controls. In contrast, higher concentrations of oPRL blocked the rise in both pFSH-R binding and P production. The alteration in pFSH-R binding was associated with a change in FSH-R number. Conclusion(s): Physiologic levels of PRL amplify the stimulatory effects of FSH on the acquisition of the FSH-R and P production in cultured granulosa cells. Higher concentrations of PRL cause a decrease in FSH-R binding and P production. Prolactin may act as a "co-gonadotropin" and fine-tune the process of folliculogenesis by altering the acquisition of granulosa FSH receptors. |
| Databáze: | OpenAIRE |
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