Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
| Autor: | Pedro Henrique Hermes de Araújo, Maria Cláudia Santos-Silva, Enio Lima Junior, Maria Eliane Merlin Rocha, Gustavo Rodrigues Rossi, Eduardo Ricci-Júnior, Amanda Virtuoso Jacques, Paulo Emilio Feuser, Claudia Sayer, Juan Marcelo Carpio Arévalo, Edvaldo S. Trindade |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Erythrocytes
Biocompatible Materials 02 engineering and technology 01 natural sciences HeLa chemistry.chemical_compound Mice Spectroscopy Fourier Transform Infrared Zeta potential Methyl methacrylate Cytotoxicity Magnetite Nanoparticles Drug Carriers biology Temperature 021001 nanoscience & nanotechnology Endocytosis Biochemistry Folate receptor visual_art Thermogravimetry Otras Ingeniería Química visual_art.visual_art_medium 0210 nano-technology Superparamagnetism Materials science Cell Survival Surface Properties Biomedical Engineering Biophysics Bioengineering INGENIERÍAS Y TECNOLOGÍAS 010402 general chemistry Hemolysis Cell Line Biomaterials Polimeric Nanoparticles Folic Acid Gallic Acid Animals Humans Polymethyl Methacrylate Particle Size technology industry and agriculture biology.organism_classification Poly(methyl methacrylate) 0104 chemical sciences Ingeniería Química purl.org/becyt/ford/2.4 [https] Kinetics purl.org/becyt/ford/2 [https] chemistry Surface modification Nuclear chemistry HeLa Cells |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| DOI: | 10.1007/s10856-016-5796-0 |
| Popis: | Lauryl gallate loaded in superparamagnetic poly (methyl methacrylate) nanoparticles surface modified with folic acid were synthesized by miniemulsion polymerization in just one step. In vitro biocompatibility and cytotoxicity assays on L929 (murine fibroblast), human red blood, and HeLa (uterine colon cancer) cells were performed. The effect of folic acid at the nanoparticles surface was evaluated through cellular uptake assays in HeLa cells. Resultsshowed that the presence of folic acid did not affect substantially the polymer particle size (~120 nm), the superparamagnetic behavior, the encapsulation efficiency of lauryl gallate (~87 %), the Zeta potential (~38 mV) of thepolymeric nanoparticles or the release profile of lauryl gallate. The release profile of lauryl gallate from superparamagnetic poly(methyl methacrylate) nanoparticles presented an initial burst effect (0?1 h) followed by a slow andsustained release, indicating a biphasic release system.Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles with folic acid did not present cytotoxicity effects on L929 and human red blood cells. However, free lauryl gallate presented significant cytotoxic effects on L929 and human red blood cells at all tested concentrations. The presence of folic acid increased the cytotoxicity of lauryl gallate loaded in nanoparticles on HeLa cells due to a higher cellular uptake when HeLa cells were incubated at 37 °C. On the other hand, when the nanoparticles were incubated at low temperature (4 °C) cellular uptake was not observed, suggesting that the uptake occurred by folate receptor mediated energy-dependent endocytosis. Based on presented results our work suggeststhat this carrier system can be an excellent alternative in targeted drug delivery by folate receptor. Fil: Feuser, Paulo Emilio. Universidade Federal de Santa Catarina; Brasil Fil: Carpio Arévalo, Juan Marcelo. Universidade Federal do Paraná; Brasil Fil: Lima, Enio Junior. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia de Física. Laboratorio de Resonancias Magnéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina Fil: Rodrigues Rossi, Gustavo. Universidade Federal do Paraná; Brasil Fil: Trindade, Edvaldo da Silva. Universidade Federal do Paraná; Brasil Fil: Merlin Rocha, Maria Eliane. Universidade Federal do Paraná; Brasil Fil: Virtuoso Jacques, Amanda. Universidade Federal de Santa Catarina; Brasil Fil: Ricci Júnior, Eduardo. Universidade Federal do Rio de Janeiro; Brasil Fil: Santos Silva, Maria Claudia. Universidade Federal de Santa Catarina; Brasil Fil: Sayer, Claudia. Universidade Federal de Santa Catarina; Brasil Fil: Hermes de Araújo, Pedro H.. Universidade Federal de Santa Catarina; Brasil |
| Databáze: | OpenAIRE |
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