Corynebacterium pseudotuberculosis may be under anagenesis and biovar Equi forms biovar Ovis: a phylogenic inference from sequence and structural analysis
| Autor: | Syed Babar Jamal, Debmalya Barh, Elaine Maria Seles Dorneles, Alberto Oliveira, Andrey Pereira Lage, Dionei Joaquim Haas, Henrique César Pereira Figueiredo, Pammella Teixeira, Marcela Santiago Pacheco de Azevedo, Preetam Ghosh, Rafaela Salgado Ferreira, Sintia Almeida, Artur Silva, Vasco Azevedo, Sandeep Tiwari, Marcos Bryan Heinemann |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Evolution Corynebacterium pseudotuberculosis Biovar animal diseases 030106 microbiology Molecular phylogeny Biology Microbiology Conserved sequence Evolution Molecular 03 medical and health sciences GENÉTICA BACTERIANA Bacterial Proteins Phylogenetics Animals Point Mutation Horses Conserved Sequence Phylogeny Nitrates Sheep Molecular epidemiology Phylogenetic tree Goats food and beverages Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification rpoB bacterial infections and mycoses Anagenesis 030104 developmental biology bacteria Cattle Structural biology Research Article |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP BMC Microbiology |
| Popis: | Background Corynebacterium pseudotuberculosis can be classified into two biovars or biovars based on their nitrate-reducing ability. Strains isolated from sheep and goats show negative nitrate reduction and are termed biovar Ovis, while strains from horse and cattle exhibit positive nitrate reduction and are called biovar Equi. However, molecular evidence has not been established so far to understand this difference, specifically if these C. pseudotuberculosis strains are under an evolutionary process. Results The ERIC 1 + 2 Minimum-spanning tree from 367 strains of C. pseudotuberculosis showed that the great majority of biovar Ovis strains clustered together, but separately from biovar Equi strains that also clustered amongst themselves. Using evolutionarily conserved genes (rpoB, gapA, fusA, and rsmE) and their corresponding amino acid sequences, we analyzed the phylogenetic relationship among eighteen strains of C. pseudotuberculosis belonging to both biovars Ovis and Equi. Additionally, conserved point mutation based on structural variation analysis was also carried out to elucidate the genotype-phenotype correlations and speciation. We observed that the biovars are different at the molecular phylogenetic level and a probable anagenesis is occurring slowly within the species C. pseudotuberculosis. Conclusions Taken together the results suggest that biovar Equi is forming the biovar Ovis. However, additional analyses using other genes and other bacterial strains are required to further support our anagenesis hypothesis in C. pseudotuberculosis. Electronic supplementary material The online version of this article (doi:10.1186/s12866-016-0717-4) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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