Genome editing by natural and engineered CRISPR-associated nucleases
| Autor: | Wu, Wen Y., Lebbink, Joyce H.G., Kanaar, Roland, Geijsen, Niels, Van Der Oost, John, Onderzoek, dCSCA RMSC-1 |
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| Přispěvatelé: | Molecular Genetics, Radiotherapy, Hubrecht Institute for Developmental Biology and Stem Cell Research, Onderzoek, dCSCA RMSC-1 |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene Editing Nuclease biology Cas9 Functional features Cell Biology Computational biology Microbiology 03 medical and health sciences 030104 developmental biology Genome editing Microbiologie Exoribonucleases biology.protein Life Science CRISPR CRISPR-Cas Systems Molecular Biology VLAG |
| Zdroj: | Nature Chemical Biology, 14(7), 642-651. Nature Publishing Group Nature Chemical Biology, 14(7), 642-651 Nature Chemical Biology 14 (2018) 7 Nature Chemical Biology, 14(7), 642. Nature Publishing Group |
| ISSN: | 1552-4450 |
| Popis: | Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity. |
| Databáze: | OpenAIRE |
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