Testosterone-induced modulation of peroxisomal morphology and peroxisome-related gene expression in brown trout (Salmo trutta f. fario) primary hepatocytes
| Autor: | Fernanda Malhão, Eduardo Rocha, Ivone Pinheiro, Célia Lopes, Tânia Vieira Madureira, L. Filipe C. Castro, Cláudia Guimarães, José Gonçalves |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Urate Oxidase Trout medicine.drug_class Health Toxicology and Mutagenesis Down-Regulation Estrogen receptor Endocrine Disruptors 010501 environmental sciences Aquatic Science 01 natural sciences Vitellogenins 03 medical and health sciences Vitellogenin Internal medicine Gene expression Peroxisomes medicine Animals PPAR alpha Testosterone Receptor Fulvestrant 0105 earth and related environmental sciences Estradiol biology Estrogen Antagonists Urate oxidase Androgen Antagonists Peroxisome Catalase Androgen Flutamide Up-Regulation 030104 developmental biology Endocrinology Hepatocytes biology.protein Water Pollutants Chemical Signal Transduction |
| Zdroj: | CIÊNCIAVITAE |
| ISSN: | 0166-445X |
| Popis: | Disruption of androgenic signaling has been linked to possible cross-modulation with other hormone-mediated pathways. Therefore, our objective was to explore effects caused by testosterone – T (1, 10 and 50 μM) in peroxisomal signaling of brown trout hepatocytes. To study the underlying paths involved, several co-exposure conditions were tested, with flutamide – F (anti-androgen) and ICI 182,780 – ICI (anti-estrogen). Molecular and morphological approaches were both evaluated. Peroxisome proliferator-activated receptor alpha (PPARα), catalase and urate oxidase were the selected targets for gene expression analysis. The vitellogenin A gene was also included as a biomarker of estrogenicity. Peroxisome relative volumes were estimated by immunofluorescence, and transmission electron microscopy was used for qualitative morphological control. The single exposures of T caused a significant down-regulation of urate oxidase (10 and 50 μM) and a general up-regulation of vitellogenin. A significant reduction of peroxisome relative volumes and smaller peroxisome profiles were observed at 50 μM. Co-administration of T and ICI reversed the morphological modifications and vitellogenin levels. The simultaneous exposure of T and F caused a significant and concentration-dependent diminishing in vitellogenin expression. Together, the findings suggest that in the tested model, T acted via both androgen and estrogen receptors to shape the peroxisomal related targets. |
| Databáze: | OpenAIRE |
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