A catechin nanoformulation inhibits WM266 melanoma cell proliferation, migration and associated neo-angiogenesis
Autor: | Liron Berger, Silke Hampel, Martina Giannaccini, Matteo Battaglini, Vittoria Raffa, Luciana Dente, Orazio Vittorio, Giuseppe Cirillo, Nicoletta di Leo |
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Rok vydání: | 2017 |
Předmět: |
(+)-catechin
0301 basic medicine Cell Pharmaceutical Science Angiogenesis Inhibitors Catechin Anti-cancer activity 0302 clinical medicine Cell Movement Phytogenic Cytotoxic T cell Melanoma Zebrafish Tumor Neovascularization Pathologic biology Chemistry General Medicine medicine.anatomical_structure Biochemistry 030220 oncology & carcinogenesis Melanoma cell line WM266 Intracellular Biotechnology Drug Compounding Carbon nanotubes Motility Antineoplastic Agents Cell Line 03 medical and health sciences Cell Line Tumor medicine Animals Humans Anti-angiogenic activity Neovascularization Cell Proliferation Pathologic Cell growth medicine.disease biology.organism_classification Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays In vitro 030104 developmental biology Nanoparticles 3003 Cancer research |
Zdroj: | European Journal of Pharmaceutics and Biopharmaceutics. 114:1-10 |
ISSN: | 0939-6411 |
Popis: | We validated the anticancer potential of a nanoformulation made by (+)-catechin, gelatin and carbon nanotubes in terms of inhibition of cancer cell proliferation, migration and associated neo-angiogenesis. Gelatin was selected to stabilize the catechin without compromising its anti-oxidant potential and the carbon nanotubes were used to increase its intracellular bioavailability. The anticancer potential of the resulting nanohybrid was validated on an aggressive melanoma cell line, in vitro and in zebrafish xenotransplants. The nanohybrid strongly enhances the cytotoxic effect of (+)-catechin. At a concentration of (+)-catechin 50μg/ml, the nanohybrid inhibited the ability of melanoma cells to proliferate (100% increase of cell doubling time and severe impairment in zebrafish xenotransplants), to migrate (totally inhibition in vitro and 50% reduction of cell motility in zebrafish xenotransplants) and to induce neo-angiogenesis (100% inhibition in zebrafish xenotransplants). Conversely, the free (+)-catechin and carrier (CNT:gel) had no statistically significant effects over the control, at any concentration tested. Our results suggest that the use of the nanohybrid, able to improve the therapeutic efficacy of the catechins, could represent a successful strategy for a future clinical translation. |
Databáze: | OpenAIRE |
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