A catechin nanoformulation inhibits WM266 melanoma cell proliferation, migration and associated neo-angiogenesis

Autor: Liron Berger, Silke Hampel, Martina Giannaccini, Matteo Battaglini, Vittoria Raffa, Luciana Dente, Orazio Vittorio, Giuseppe Cirillo, Nicoletta di Leo
Rok vydání: 2017
Předmět:
(+)-catechin
0301 basic medicine
Cell
Pharmaceutical Science
Angiogenesis Inhibitors
Catechin
Anti-cancer activity
0302 clinical medicine
Cell Movement
Phytogenic
Cytotoxic T cell
Melanoma
Zebrafish
Tumor
Neovascularization
Pathologic
biology
Chemistry
General Medicine
medicine.anatomical_structure
Biochemistry
030220 oncology & carcinogenesis
Melanoma cell line WM266
Intracellular
Biotechnology
Drug Compounding
Carbon nanotubes
Motility
Antineoplastic Agents
Cell Line
03 medical and health sciences
Cell Line
Tumor
medicine
Animals
Humans
Anti-angiogenic activity
Neovascularization
Cell Proliferation
Pathologic
Cell growth
medicine.disease
biology.organism_classification
Antineoplastic Agents
Phytogenic
Xenograft Model Antitumor Assays
In vitro
030104 developmental biology
Nanoparticles
3003
Cancer research
Zdroj: European Journal of Pharmaceutics and Biopharmaceutics. 114:1-10
ISSN: 0939-6411
Popis: We validated the anticancer potential of a nanoformulation made by (+)-catechin, gelatin and carbon nanotubes in terms of inhibition of cancer cell proliferation, migration and associated neo-angiogenesis. Gelatin was selected to stabilize the catechin without compromising its anti-oxidant potential and the carbon nanotubes were used to increase its intracellular bioavailability. The anticancer potential of the resulting nanohybrid was validated on an aggressive melanoma cell line, in vitro and in zebrafish xenotransplants. The nanohybrid strongly enhances the cytotoxic effect of (+)-catechin. At a concentration of (+)-catechin 50μg/ml, the nanohybrid inhibited the ability of melanoma cells to proliferate (100% increase of cell doubling time and severe impairment in zebrafish xenotransplants), to migrate (totally inhibition in vitro and 50% reduction of cell motility in zebrafish xenotransplants) and to induce neo-angiogenesis (100% inhibition in zebrafish xenotransplants). Conversely, the free (+)-catechin and carrier (CNT:gel) had no statistically significant effects over the control, at any concentration tested. Our results suggest that the use of the nanohybrid, able to improve the therapeutic efficacy of the catechins, could represent a successful strategy for a future clinical translation.
Databáze: OpenAIRE
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