| Autor: |
Daphne F. Rush, Andrew L. Kung, Robert T. Schimke, Steven W. Sherwood |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Experimental Cell Research. 211:275-281 |
| ISSN: |
0014-4827 |
| Popis: |
Using a procedure to stain cells simultaneously for cyclin B1 protein and DNA, we have examined cyclin B1 expression by flow cytometry in human cells under a variety of perturbing and nonperturbing conditions. The method described is useful for measuring relative differences in cyclin B level (immunochemically detectable epitope) as a function of cell cycle position on an individual cell basis and thus to examine cell cycle-related changes in cyclin B expression without prior cell synchronization. We show that in HeLa S3 cells, cyclin B1 accumulates in cells only after they become 4C and have resided in G2 for a short period of time. During colcemid-induced mitotic arrest cyclin B1 continues to accumulate in HeLa S3 cells, and under specific conditions of aphidicolin-induced unbalanced cell growth induced, cyclin B accumulates to supranormal levels prior to mitosis. Flow cytometric analysis of cyclin B expression and DNA content permits detailed examination of the effects of cell cycle perturbations on cyclin B expression under a variety of conditions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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