Targeted Treatment of Ischemic Stroke by Bioactive Nanoparticle-Derived Reactive Oxygen Species Responsive and Inflammation-Resolving Nanotherapies
| Autor: | Jialu Huang, Hong Ran, Jichao Yuan, Lan Wen, Linke Zhou, Kaiyao Hu, Lanlan Li, Xiaoxing Xiong, Ying Jiang, Qinghua Yang, Jie Wang, Zhenhua Zhou, Jianxiang Zhang, Wendan Pu |
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| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
medicine.medical_treatment General Physics and Astronomy Inflammation Oxidative phosphorylation Pharmacology Brain Ischemia Pathogenesis Mice In vivo medicine Distribution (pharmacology) Animals General Materials Science Stroke Ischemic Stroke chemistry.chemical_classification Reactive oxygen species Chemistry General Engineering medicine.disease Nanoparticles medicine.symptom Reactive Oxygen Species |
| Zdroj: | ACS nano. 15(10) |
| ISSN: | 1936-086X |
| Popis: | Stroke is a primary cause of death and disability worldwide, while effective and safe drugs remain to be developed for its clinical treatment. Herein, we report bioactive nanoparticle-derived multifunctional nanotherapies for ischemic stroke, which are engineered from a pharmacologically active oligosaccharide material (termed as TPCD) prepared by covalently conjugating a radical-scavenging compound (Tempol) and a hydrogen-peroxide-eliminating moiety of phenylboronic acid pinacol ester (PBAP) on β-cyclodextrin. Of note, combined functional moieties of Tempol and PBAP on β-cyclodextrin contribute to antioxidative and anti-inflammatory activities of TPCD. Cellularly, TPCD nanoparticles (i.e., TPCD NPs) reduced oxygen-glucose deprivation-induced overproduction of oxidative mediators, increased antioxidant enzyme expression, and suppressed microglial-mediated inflammation, thereby inhibiting neuronal apoptosis. After intravenous (i.v.) delivery, TPCD NPs could efficiently accumulate at the cerebral ischemic injury site of mice with middle cerebral artery occlusion (MCAO), showing considerable distribution in cells relevant to the pathogenesis of stroke. Therapeutically, TPCD NPs significantly decreased infarct volume and accelerated recovery of neurological function in MCAO mice. Mechanistically, efficacy of TPCD NPs is achieved by its antioxidative, anti-inflammatory, and antiapoptotic effects. Furthermore, TPCD NPs can function as a reactive oxygen species labile nanovehicle to efficiently load and triggerably release an inflammation-resolving peptide Ac2-26, giving rise to an inflammation-resolving nanotherapy (i.e., ATPCD NP). Compared to TPCD NP, ATPCD NP demonstrated notably enhanced in vivo efficacies, largely resulting from its additional inflammation-resolving activity. Consequently, TPCD NP-derived nanomedicines can be further developed as promising targeted therapies for stroke and other inflammation-associated cerebrovascular diseases. |
| Databáze: | OpenAIRE |
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