Electroacupuncture potentiates peripheral CB2 receptor-inhibited chronic pain in a mouse model of knee osteoarthritis
| Autor: | Jing-Jing Li, Jie Cao, Man Li, He Zhu, Cai-Hua Wu, Hong-Ping Li, Wen Su, Hong-Chun Xiang, Xuefei Hu, Qiang Wang, Xiao-Cui Yuan, Li-Xue Lin, Fang Gao |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Electroacupuncture medicine.medical_treatment Osteoarthritis 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Acupuncture Cannabinoid receptor type 2 Journal of Pain Research Hot plate test Original Research inflammatory pain business.industry Chronic pain cannabinoid medicine.disease Peripheral 030104 developmental biology Anesthesiology and Pain Medicine Endocrinology IL-1β lipids (amino acids peptides and proteins) Cannabinoid business acupuncture 030217 neurology & neurosurgery |
| Zdroj: | Journal of Pain Research |
| ISSN: | 1178-7090 |
| DOI: | 10.2147/jpr.s171664 |
| Popis: | Xiao-Cui Yuan,1 Qiang Wang,2 Wen Su,3 Hong-Ping Li,1 Cai-Hua Wu,3 Fang Gao,1 Hong-Chun Xiang,1 He Zhu,1 Li-Xue Lin,1 Xue-Fei Hu,1 Jie Cao,4 Jing-Jing Li,1 Man Li1 1Department of Neurobiology and Key Laboratory of Neurological Diseases of Ministry of Education, The Institute of Brain Research, School of Basic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, People’s Republic of China; 2Department of Anesthesiology, First Affiliated Hospital of Xi’an JiaoTong University, Xi’an, People’s Republic of China; 3Department of Acupuncture, Wuhan First Hospital, Wuhan 430030, People’s Republic of China; 4Department of Neurology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, People’s Republic of China Purpose: Knee osteoarthritis (KOA) is a highly prevalent, chronic joint disorder, with chronic pain as its typical symptom. Although studies have shown that an activated peripheral CB2 receptor can reduce acute pain, whether the CB2 receptor is involved in electroacupuncture (EA) inhibiting chronic pain and the involved mechanism remains unclear. The aim of this study was to investigate whether EA may strengthen peripheral CB2 receptor-inhibited chronic pain in a mouse model of KOA. Materials and methods: KOA was induced by intra-articular injection of monosodium iodoacetate (MIA) into the left knee joint of mice. Thermal hyperalgesia was tested with the hot plate test, and mechanical allodynia was quantified using von Frey filaments. The expression of CB2 receptor and IL-1β were quantified by using immunofluorescence labeling. Results: EA treatment at 2Hz+1mA significantly increased the expression of CB2 receptor in fibroblasts and decreased the expression of IL-1β in the menisci compared with that in the KOA group. However, EA had no effect on the expression of IL-1β in CB2−/− mice. At 2Hz+1mA, EA significantly increased mechanical threshold, thermal latency, and weight borne after KOA modeling. However, knockout of the CB2 receptor blocked these effects of EA. After 2Hz+1mA treatment, EA significantly reduced the Osteoarthritis Research Society International (OARSI) score after KOA modeling. However, EA had no significant effect on the OARSI score in CB2−/− mice. Conclusion: EA reduced the expression of IL-1β by activating the CB2 receptor, thus inhibiting the chronic pain in the mouse model of KOA. Keywords: cannabinoid, acupuncture, inflammatory pain, IL-1&beta |
| Databáze: | OpenAIRE |
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