Detoxification of Aflatoxin-Contaminated Maize by Neutral Electrolyzed Oxidizing Water
| Autor: | Alicia Marroquin-Cardona, Tania Villarreal-Barajas, Abraham Méndez-Albores, Roberto Díaz-Torres, Samantha Jardon-Xicotencatl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Salmonella typhimurium
Aflatoxin aflatoxins Cell Survival Health Toxicology and Mutagenesis lcsh:Medicine Food Contamination Aspergillus flavus Toxicology medicine.disease_cause maize Zea mays Electrolysis Article Ames test Lipid peroxidation chemistry.chemical_compound medicine neutral electrolyzed oxidizing water Humans heterocyclic compounds Lymphocytes Food science detoxification Mycotoxin Carcinogen Dose-Response Relationship Drug biology Chemistry lcsh:R technology industry and agriculture Water food and beverages Hep G2 Cells Oxidants biology.organism_classification Comet assay Environmental chemistry Comet Assay Lipid Peroxidation Genotoxicity DNA Damage |
| Zdroj: | Toxins, Vol 7, Iss 10, Pp 4294-4314 (2015) Toxins Volume 7 Issue 10 Pages 4294-4314 |
| ISSN: | 2072-6651 |
| Popis: | Aflatoxins, a group of extremely toxic mycotoxins produced by Aspergillus flavus, A. parasiticus and A. nomius, can occur as natural contaminants of certain agricultural commodities, particularly maize. These toxins have been shown to be hepatotoxic, carcinogenic, mutagenic and cause severe human and animal diseases. The effectiveness of neutral electrolyzed oxidizing water (NEW) on aflatoxin detoxification was investigated in HepG2 cells using several validation methodologies such as the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay, the induction of lipid peroxidation, the oxidative damage by means of glutathione modulation, the Ames test and the alkaline Comet assay. Our results showed that, after the aflatoxin-contaminated maize containing 360 ng/g was soaked in NEW (60 mg/L available chlorine, pH 7.01) during 15 min at room temperature, the aflatoxin content did not decrease as confirmed by the immunoaffinity column and ultra performance liquid chromatography methods. Aflatoxin fluorescence strength of detoxified samples was similar to untreated samples. However, aflatoxin-associated cytotoxicity and OPEN ACCESS Toxins 2015, 7 4295 genotoxicity effects were markedly reduced upon treatment. According to these results, NEW can be effectively used to detoxify aflatoxin-contaminated maize. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|