Natural variation in a type-A response regulator confers maize chilling tolerance
| Autor: | Zhuoyang Li, Pan Yin, Jinkui Cheng, Caifu Jiang, Diyi Fu, Yiting Shi, Shuhua Yang, Rong Zeng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Agricultural genetics
Science General Physics and Astronomy In Vitro Techniques Biology Genes Plant Models Biological Zea mays Article General Biochemistry Genetics and Molecular Biology Transcriptome Inbred strain Gene Expression Regulation Plant Stress Physiological Genetic variation Phosphorylation Protein kinase A Gene Alleles Plant Proteins Regulation of gene expression Multidisciplinary Abiotic Gene Expression Profiling fungi Genetic Variation food and beverages General Chemistry Plants Genetically Modified Recombinant Proteins Cell biology Cold Temperature Response regulator Natural variation in plants Glucosyltransferases Mitogen-Activated Protein Kinases |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Maize (Zea mays L.) is a cold-sensitive species that often faces chilling stress, which adversely affects growth and reproduction. However, the genetic basis of low-temperature adaptation in maize remains unclear. Here, we demonstrate that natural variation in the type-A Response Regulator 1 (ZmRR1) gene leads to differences in chilling tolerance among maize inbred lines. Association analysis reveals that InDel-35 of ZmRR1, encoding a protein harboring a mitogen-activated protein kinase (MPK) phosphorylation residue, is strongly associated with chilling tolerance. ZmMPK8, a negative regulator of chilling tolerance, interacts with and phosphorylates ZmRR1 at Ser15. The deletion of a 45-bp region of ZmRR1 harboring Ser15 inhibits its degradation via the 26 S proteasome pathway by preventing its phosphorylation by ZmMPK8. Transcriptome analysis indicates that ZmRR1 positively regulates the expression of ZmDREB1 and Cellulose synthase (CesA) genes to enhance chilling tolerance. Our findings thus provide a potential genetic resource for improving chilling tolerance in maize. The genetic basis of low-temperature tolerance in maize is unclear. Here, the authors show that the type-A Response Regulator 1 (ZmRR1) and mitogen-activated protein kinase (MPK8) are positive and negative regulators of maize chilling tolerance, and ZmRR1 is phosphorylated by ZmMPK8 during cold treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink