Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine
| Autor: | Harald Pichler, Sarah Waldie, Michael Haertlein, Martine Moulin, Tania Kjellerup Lind, Selma Maric, V. Trevor Forsyth, Kathryn L. Browning, Marité Cárdenas, Gernot Strohmeier, Alessandra Luchini |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lipid Bilayers 02 engineering and technology Neutron scattering 03 medical and health sciences chemistry.chemical_compound Phosphatidylcholine Electrochemistry Molecule General Materials Science Lipid bilayer Spectroscopy Chemistry Cholesterol Bilayer Surfaces and Interfaces Deuterium 021001 nanoscience & nanotechnology Condensed Matter Physics 030104 developmental biology Membrane Phosphatidylcholines Biophysics lipids (amino acids peptides and proteins) 0210 nano-technology |
| Popis: | Cholesterol is an essential component of mammalian membranes and is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid-ordered phases with an increased thickness and a configurational order as compared to liquid-disordered phases. For saturated lipid membranes, cholesterol molecules localize close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote relocation of cholesterol toward the inner interface between the two bilayer leaflets. Here, neutron reflection is used to study the location of cholesterol (both non-deuterated and per-deuterated versions are used) within supported lipid bilayers composed of a natural mixture of phosphatidylcholine (PC). The lipids were produced in a genetically modified strain of Escherichia coli and grown under specific deuterated conditions to give an overall neutron scattering length density (which depends on the level of deuteration) of the lipids matching that of D |
| Databáze: | OpenAIRE |
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