| Autor: |
Degtyareva, N P, Greenwell, P, Hofmann, E R, Hengartner, M O, Zhang, L, Culotti, J G, Petes, T D |
| Přispěvatelé: |
University of Zurich, Petes, T D |
| Rok vydání: |
2002 |
| Předmět: |
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| DOI: |
10.5167/uzh-947 |
| Popis: |
Mismatch repair genes are important in maintaining the fidelity of DNA replication. To determine the function of the Caenorhabditis elegans homologue of the MSH2 mismatch repair gene (msh-2), we isolated a strain of C. elegans with an insertion of the transposable element Tc1 within msh-2. Early-passage msh-2 mutants were similar to wild-type worms with regard to lifespan and meiotic chromosome segregation but had slightly reduced fertility. The mutant worms had reduced DNA damage-induced germ-line apoptosis after genotoxic stress. The msh-2 mutants also had elevated levels of microsatellite instability and increased rates of reversion of the dominant unc-58(e665) mutation. In addition, serially passaged cultures of msh-2 worms died out much more quickly than those of wild-type worms. These results demonstrate that msh-2 function in C. elegans is important in regulating both short- and long-term genomic stability. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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