Characterization and immuno-detection of AIDA-I adhesin isolated from porcine Escherichia coli
| Autor: | Elemir Simko, Y. Fang, Musangu Ngeleka, Dorothy M. Middleton |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Gene isoform
Swine Blotting Western Molecular Sequence Data medicine.disease_cause Sensitivity and Specificity Microbiology Sequence Analysis Protein Agglutination Tests Immuno detection Escherichia coli medicine Animals Humans Protein Isoforms Electrophoresis Gel Two-Dimensional Amino Acid Sequence Gene Escherichia coli Infections Swine Diseases Adhesins Escherichia coli General Veterinary biology General Medicine biology.organism_classification Molecular biology Enterobacteriaceae Molecular Weight Bacterial adhesin Diarrhea Electrophoresis Polyacrylamide Gel medicine.symptom Sequence Alignment Bacteria |
| Zdroj: | Veterinary Microbiology. 109:65-73 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2005.04.018 |
| Popis: | A relatively high percentage of porcine Escherichia coli isolates from cases associated with neonatal and post-weaning diarrhea are positive for the gene encoding adhesin involved in diffuse adhesion I (AIDA-I). This gene and its corresponding protein were first identified and characterized in E. coli strain 2787 isolated from human infantile diarrhea. Little is known about the properties of the AIDA-I protein and its immuno-detection on surface of AIDA-I positive porcine E. coli isolates. In this study, we demonstrated that the AIDA-I adhesin isolated from porcine AIDA-I positive E. coli is an acidic protein consisting of five isoforms. It has a similar molecular weight (100 kDa) and relatively high amino acid homology (78–87%) with the AIDA-I adhesin expressed by human AIDA-I positive E. coli strain 2787. Based on limited comparison, it appears that there is a very high homology among AIDA-I proteins expressed by porcine AIDA-I positive E. coli isolates. Sensitivity of detection of surface AIDA-I adhesin of PCR-positive AIDA-I E. coli by immuno-dot-blot and coagglutination tests was 76 and 71%, respectively, whereas specificity was 89 and 84%, respectively. These tests are unlikely to be used for diagnostic detection of AIDA-I positive E. coli due to the relatively low sensitivity; however, they may be potentially useful for identification of false positive reactions generated by other diagnostic tests. |
| Databáze: | OpenAIRE |
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