An ultrasensitive bunge bedstraw herb type DNA machine for absolute quantification of mRNA in single cell
| Autor: | Shulin Zhao, Chunhuan Xu, Jingjin Zhao, Shengyu Chen, Ivan Sakharov, Shengqiang Hu |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
History
Polymers and Plastics Survivin Biomedical Engineering Biophysics Nucleic Acid Hybridization Biosensing Techniques DNA DNA Catalytic General Medicine Industrial and Manufacturing Engineering Galium Limit of Detection Electrochemistry RNA Messenger Business and International Management Nucleic Acid Amplification Techniques Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 217:114702 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2022.114702 |
| Popis: | Messenger ribonucleic acids (mRNAs) comprise a class of small nucleic acids carrying genetic information, which exhibit very important role in medical research and diagnosis. If only the mean mRNA expression levels of the mRNA population are considered in medical research, important information linking mRNA expression and cellular function may be lost. Single-cell analysis provides valuable insights into studying its heterogeneity, signaling, and stochastic gene expression. In this study, a "bunge bedstraw herb"-type DNA machine based on DNAzyme catalyzing coupled clamping hybrid chain reaction (c-HCR) is presented. In the DNA machine, a bunge bedstraw herb-type DNA structure was first formed by hybridizing a core junction scaffold cruciform probe to a hairpin probe that can trigger the c-HCR via a target molecule in four directions. This approach can reduce the detection limit of mRNA to 5 × 10 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect