Flubendazole residues in eggs after oral administration to laying hens: determination with reversed phase liquid chromatography
| Autor: | H. J. Keukens, M. J. H. Tomassen, C.A. Kan |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
food.ingredient
Metabolite Eggs Flubendazole Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound food Egg White Oral administration Yolk Electrochemistry medicine Life Science Environmental Chemistry Animals Anthelmintic Instituut voor Dierhouderij en Diergezondheid Spectroscopy Residue (complex analysis) Chromatography ID-Lelystad Wageningen Food Safety Research Antinematodal Agents Egg Yolk Drug Residues ID Lelystad Mebendazole chemistry ID-Lelystad Instituut voor Dierhouderij en Diergezondheid ID Lelystad Institute for Animal Science and Health embryonic structures Female Chickens Institute for Animal Science and Health medicine.drug Egg white Chromatography Liquid |
| Zdroj: | The Analyst, 123, 2525-2527 The Analyst 123 (1998) |
| ISSN: | 0003-2654 |
| Popis: | Flubendazole residues in eggs were experimentally induced by providing groups of 8 laying hens feed with approximately 3, 10 and 30 mg kg-1 flubendazole for 21 days. Eggs were sampled during this period and one week after the administration. Samples of both whole egg and egg white/yolk were analysed separately. Flubendazole analysis was performed by reversed phase HPLC and UV detection at 250 nm (eggs) or 320 nm (feed). The limit of detection (LOD) for flubendazole in feed was 0.3 mg kg-1 and in whole egg 0.012 mg kg-1. Both the hydrolysed and reduced metabolites of flubendazole were also determined quantitatively. The eggs of control hens housed in the same room during the study period did not contain any detectable flubendazole or metabolite residue. The eggs from the lowest dosed group (3 mg kg-1 feed) did contain residues, but most of them were only slightly higher than the LOD. Residues in eggs collected from the laying hens that obtained feed with 10 and 30 mg kg-1 flubendazole reached a plateau level after some 10 days and there was a good dose response relation between levels in feed and those in eggs. The residues of parent compound and metabolites almost exclusively occurred in yolk, the metabolites accounting for some 60-65% of the total residue. The residues of the parent compound and its metabolites declined below 100 micrograms kg-1 5 days after the administration of dosed feed had ended. |
| Databáze: | OpenAIRE |
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