Adipose tissue-derived stem cells reduce acute and chronic kidney damage in mice
| Autor: | Alvaro Pacheco-Silva, Priscilla Barbosa Costa, Marina Burgos-Silva, Marco Antonio Cenedeze, Cassiano Donizetti-Oliveira, Niels Olsen Saraiva Câmara, Meire Ioshie Hiyane, Patricia Semedo-Kuriki |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_treatment Adipose tissue lcsh:Medicine Inflammation Kidney Mice Folic Acid Fibrosis medicine Animals Urea lcsh:Science Cells Cultured Multidisciplinary business.industry Stem Cells Mesenchymal stem cell lcsh:R Acute kidney injury RIM Stem-cell therapy Acute Kidney Injury medicine.disease Disease Models Animal medicine.anatomical_structure Adipose Tissue Immunology Chronic Disease Cancer research lcsh:Q medicine.symptom Stem cell business Research Article Stem Cell Transplantation |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP PLoS ONE, Vol 10, Iss 11, p e0142183 (2015) PLoS ONE |
| Popis: | Acute and chronic kidney injuries (AKI and CKI) constitute syndromes responsible for a large part of renal failures, and are today still associated with high mortality rates. Given the lack of more effective therapies, there has been intense focus on the use stem cells for organ protective and regenerative effects. Mesenchymal stem cells (MSCs) have shown great potential in the treatment of various diseases of immune character, although there is still debate on its mechanism of action. Thus, for a greater understanding of the role of MSCs, we evaluated the effect of adipose tissue-derived stem cells (AdSCs) in an experimental model of nephrotoxicity induced by folic acid (FA) in FVB mice. AdSC-treated animals displayed kidney functional improvement 24h after therapy, represented by reduced serum urea after FA. These data correlated with cell cycle regulation and immune response modulation via reduced chemokine expression and reduced neutrophil infiltrate. Long-term analyses, 4 weeks after FA, indicated that AdSC treatment reduced kidney fibrosis and chronic inflammation. These were demonstrated by reduced interstitial collagen deposition and tissue chemokine and cytokine expression. Thus, we concluded that AdSC treatment played a protective role in the framework of nephrotoxic injury via modulation of inflammation and cell cycle regulation, resulting in reduced kidney damage and functional improvement, inhibiting organ fibrosis and providing long-term immune regulation. |
| Databáze: | OpenAIRE |
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